Chlorine dioxide inhibits the replication of porcine reproductive and respiratory syndrome virus by blocking viral attachment
Chlorine dioxide inhibits the replication of porcine reproductive and respiratory syndrome virus by blocking viral attachment
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DOI：
10.1016/j.meegid.2018.11.002
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发表时间：
2019-01-01
影响因子：
3.2
通讯作者：
Guo, Chunhe
中科院分区：
文献类型：
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作者：
Zhu, Zhenbang;Guo, Yang;Guo, Chunhe
猪繁殖与呼吸综合征病毒（PRRSV）给全球养猪业造成了巨大的经济损失。目前的防治措施对控制猪繁殖与呼吸综合征（PARS）的暴发和传播效果不明显。换句话说，迫切需要新的抗病毒策略。二氧化氯（ClO2）被认为是一种广谱消毒剂，对微生物和寄生虫有很强的抑制作用。本研究的目的是评估ClO2体外对PRRSV感染的抑制作用及其潜在的分子机制。在这里，我们发现纯度为99%的ClO2可以抑制PRRSV在Marc-145细胞和猪肺泡巨噬细胞（PAMs）中的感染和复制。ClO2可以阻断PRRSV与细胞的结合，而不是内化和释放，表明ClO2阻断了病毒生命周期的第一阶段。我们还证明了ClO2的抑制作用归因于PRRSV基因组和蛋白质的降解。此外，我们证实ClO2可以降低PRRSV诱导的炎症细胞因子的表达。综上所述，ClO2是一种有效的体外抑制PRRSV感染的药物。
Porcine reproductive and respiratory syndrome virus (PRRSV) causes a great economic loss to the swine industry globally. Current prevention and treatment measures are not effective to control the outbreak and spread of porcine reproductive and respiratory syndrome (PARS). In other words, new antiviral strategies are urgently needed. Chlorine dioxide (ClO2) is regarded as a broad-spectrum disinfectant with strong inhibitory effects on microbes and parasites. The purpose of this study was to evaluate the inhibitory effects and underlying molecular mechanisms of ClO2 against PRRSV infection in vitro. Here, we identified ClO2 (the purity is 99%) could inhibit the infection and replication of PRRSV in both Marc-145 cells and porcine alveolar macrophages (PAMs). ClO2 could block PRRSV binding to cells rather than internalization and release, suggesting that ClO2 blocks the first stage of the virus life cycle. We also demonstrated that the inhibition exerted by ClO2 was attributed to the degradation of PRRSV genome and proteins. Moreover, we confirmed that ClO2 could decrease the expression of inflammatory cytokines induced by PRRSV. In summary, ClO2 is an efficient agent and potently suppressed PRRSV infection in vitro.