Effect of dietary arginine on growth, intestinal enzyme activities and gene expression in muscle, hepatopancreas and intestine of juvenile Jian carp (Cyprinus carpio var. Jian)
Effect of dietary arginine on growth, intestinal enzyme activities and gene expression in muscle, hepatopancreas and intestine of juvenile Jian carp (Cyprinus carpio var. Jian)
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DOI：
10.1017/s0007114511005459
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发表时间：
2012-07-28
影响因子：
3.6
通讯作者：
Zhou, Xiaoqiu
中科院分区：
文献类型：
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作者：
Chen, Gangfu;Feng, Lin;Zhou, Xiaoqiu
本研究是为了验证膳食精氨酸促进消化和吸收能力，从而促进鱼类生长的假设。这种改善可能与雷帕霉素（TOR）靶点和eif4e结合蛋白（4E-BP）有关。选用1200尾平均初始体重为6.33 (SE 0.03) g的建鲤幼鱼，分别饲喂精氨酸水平为9.8（对照）、12.7、16.1、18.5、21.9和24.5 g /kg的饲粮，为期9周。实时荧光定量PCR检测TOR和4E-BP在鱼肌肉、肝胰腺和肠道中的相对表达量。饲粮精氨酸水平显著提高（P < 0.05）：(1)肌肉和肝胰脏谷氨酸-草酰乙酸转氨酶和谷氨酸-丙酮酸转氨酶活性；(2)肠道和肝胰脏蛋白质含量、褶皱高度及肠道胰蛋白酶、凝乳胰蛋白酶、脂肪酶、Na+/K+- atp酶、碱性磷酸酶、γ -谷氨酰转肽酶和肌酸激酶活性；(3)乳酸菌计数；(4) TOR在肌肉、肝胰腺和远端肠（DI）中的相对表达；(5)与对照组比较，4E-BP在近端肠（PI）和中肠（MI）的相对表达量。相比之下，饲粮精氨酸降低了（P < 0.05）：(1)血浆氨含量；(2)嗜水气单胞菌、大肠杆菌计数；(3) TOR在PI和MI中的相对表达；(4) 4E-BP在肌肉、肝胰腺和DI中的相对表达。经二次回归分析，以特定生长率估算的日粮精氨酸需用量为18.0 g/kg。这些结果表明精氨酸促进了鱼的生长、消化和吸收能力，并调节了TOR和4E-BP基因的表达。
The present study was conducted to test the hypothesis that dietary arginine promotes digestion and absorption capacity, and, thus, enhances fish growth. This improvement might be related to the target of rapamycin (TOR) and eIF4E-binding protein (4E-BP). A total of 1200 juvenile Jian carp, Cyprinus carpio var. Jian, with an average initial weight of 6.33 (SE 0.03) g, were fed with diets containing graded concentrations of arginine, namely, 9.8 (control), 12.7, 16.1, 18.5, 21.9 and 24.5 g arginine/kg diet for 9 weeks. An real-time quantitative PCR analysis was performed to determine the relative expression of TOR and 4E-BP in fish muscle, hepatopancreas and intestine. Dietary arginine increased (P < 0.05): (1) glutamate-oxaloacetate transaminase and glutamate-pyruvate transaminase activities in muscle and hepatopancreas; (2) intestine and hepatopancreas protein content, folds height, and trypsin, chymotrypsin, lipase, Na+/K+-ATPase, alkaline phosphatase, gamma-glutamyl transpeptidase and creatine kinase activities in intestine; (3) Lactobacillus counts; (4) relative expression of TOR in the muscle, hepatopancreas and distal intestine (DI); (5) relative expression of 4E-BP in proximal intestine (PI) and mid-intestine (MI), as compared with the control group. In contrast, dietary arginine reduced (P < 0.05): (1) plasma ammonia content; (2) Aeromonas hydrophila and Escherichia coli counts; (3) relative expression of TOR in PI and MI; (4) relative expression of 4E-BP in the muscle, hepatopancreas and DI. The arginine requirement estimated by specific growth rate using quadratic regression analysis was found to be 18.0 g/kg diet. These results indicate that arginine improved fish growth, digestive and absorptive ability and regulated the expression of TOR and 4E-BP genes.