Polymer Nanoparticle Hydrogels with Autonomous Affinity Switching for the Protection of Proteins from Thermal Stress
Polymer Nanoparticle Hydrogels with Autonomous Affinity Switching for the Protection of Proteins from Thermal Stress
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DOI：
10.1002/anie.201404881
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发表时间：
2014-08-25
影响因子：
16.6
通讯作者：
Shea, Kenneth J.
中科院分区：
文献类型：
--
作者：
Beierle, John M.;Yoshimatsu, Keiichi;Shea, Kenneth J.
我们报告了一种新的材料设计概念的合成，热响应聚（N-异丙基丙烯酰胺）为基础的共聚物纳米粒子（NP）水凝胶，保护蛋白质免受热应力。NP水凝胶结合并保护靶酶在暴露于热时不可逆地丧失活性，但在随后冷却溶液时“自主地”释放酶。将最佳量的带负电荷和疏水性共聚单体并入NP水凝胶是实现这些期望功能的关键。由于NP水凝胶在室温下不显示对酶的强亲和力，它们可以保留在溶液中而不会不利地影响酶活性，或者它们可以通过过滤除去以将酶留在溶液中。结果表明，这种方法的承诺，提高蛋白质的耐热性。
We report a new material design concept for synthetic, thermally responsive poly(N-isopropylacrylamide)-based copolymer nanoparticle (NP) hydrogels, which protect proteins from thermal stress. The NP hydrogels bind and protect a target enzyme from irreversible activity loss upon exposure to heat but "autonomously" release the enzyme upon subsequent cooling of the solution. Incorporation of the optimized amount of negatively charged and hydrophobic comonomers to the NP hydrogels was key to achieve these desired functions. As the NP hydrogels do not show a strong affinity for the enzyme at room temperature, they can remain in solution without adversely affecting enzymatic activity or they can be removed by filtration to leave the enzyme in solution. The results demonstrate the promise of this approach for improving the thermal tolerance of proteins.