Population study and validation of paternity testing for thoroughbred horses by 15 microsatellite loci
Population study and validation of paternity testing for thoroughbred horses by 15 microsatellite loci
复制标题
DOI：
10.1292/jvms.63.1191
复制
发表时间：
2001-11-01
影响因子：
1.2
通讯作者：
Tomita, M
中科院分区：
文献类型：
--
作者：
Tozaki, T;Kakoi, H;Tomita, M
微卫星15 TKY系统的特点是亲子鉴定马登记。微卫星15 TKY系统通过使用15个微卫星TKY279、TKY287、TKY294、TKY297、TKY301、TKY312、TKY321、TKY325、TKY333、TKY337、TKY341、TKY343、TKY344、TKY374和TKY394构建，以提供特异性优化用于荧光检测的严格的基于PCR的微卫星分型。微卫星15 TKY系统对250匹无关的纯种马表现出良好的分辨率，每个微卫星的排除概率（PE）范围为0.437至0.621，导致纯种马的总PE值为99.998%。这些结果表明，微卫星15 TKY系统可用于纯种马的亲子鉴定。利用微卫星15 TKY系统对一个纯种马家系的亲权鉴定案例进行了分析。在这种情况下，微卫星15 TKY系统排除了假父系的亲子关系。结论：微卫星15 TKY系统可为亲子鉴定提供充分可靠的信息。
Microsatellite 15 TKY System was characterized for parentage verification of horse registry. The Microsatellite 15 TKY System was constructed by using 15 microsatellites, TKY279, TKY287, TKY294, TKY297, TKY301, TKY312, TKY321, TKY325, TKY333, TKY337, TKY341, TKY343, TKY344, TKY374, and TKY394, to provide stringent PCR-based microsatellite typing specifically optimized for multicolor fluorescence detection. The Microsatellite 15 TKY System showed good resolutions for 250 unrelated Thoroughbred horses, and the probability of exclusion (PE) at each microsatellite ranged from 0.437 to 0.621, resulting in a total PE value of 99.998% for Thoroughbred horses. These results indicated that the Microsatellite 15 TKY System is useful for paternity testing of Thoroughbred horses. A paternity testing case for a Thoroughbred horse family, in which candidate sires had close relations, was analyzed using the Microsatellite 15 TKY System. In this case, the Microsatellite 15 TKY System excluded paternity of a false sire. We concluded that the Microsatellite 15 TKY System can give sufficient and reliable information for paternity, testing.