A Rapid and Reliable Method for Monitoring the Sensitivity of Sclerotinia sclerotiorum to Boscalid
A Rapid and Reliable Method for Monitoring the Sensitivity of Sclerotinia sclerotiorum to Boscalid
复制标题
DOI：
10.1111/j.1439-0434.2007.01294.x
复制
发表时间：
2007-12
影响因子：
1.5
通讯作者：
G. Stammler;G. Benzinger;J. Speakman
中科院分区：
文献类型：
--
作者：
G. Stammler;G. Benzinger;J. Speakman
Boscalid是一种新的杀菌剂，已在欧洲多个国家的不同作物中发现。为了可持续地使用这种化合物，必须观察目标病原体的敏感性。为快速、可靠地测定组织培养板中菌丝生长的灵敏度，建立了液体YBA培养基中菌丝生长试验方法。对未接触过双碱的分离株和引入双碱处理后从油菜和豆类中分离的田间分离株进行了敏感性监测。所有菌株在1.0 mg有效成分/1以内均被完全抑制。未接触过的分离株和2006年收集的分离株的ED 50值频率分布相似。
Boscalid is a new fungicidal compound registered against Sclerotinia sclerotiorum in different crops in several European countries. For a sustainable use of this compound, the sensitivity of the target pathogen has to be observed. A mycelial growth test in tissue culture plates with liquid YBA medium was developed for a rapid and reliable sensitivity determination. Isolates never exposed to boscalid and field isolates from oilseed rape and beans, which had been isolated after boscalid market introduction and mainly from fields with boscalid treatments, were monitored for their sensitivity. All isolates were totally inhibited within 1.0 mg active ingredient/1. The frequency distribution of the ED 50 values was similar both for those isolates which had never been exposed to boscalid and for those isolates collected in 2006.