Transcriptome Analysis of Upland Rice in Response to PEG Stress during Seed Germination
Transcriptome Analysis of Upland Rice in Response to PEG Stress during Seed Germination
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旱稻种子萌发过程中 PEG 胁迫响应的转录组分析
DOI：
10.17957/ijab/15.1202
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发表时间：
2019-12
影响因子：
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通讯作者：
Quanzhi Zhao
中科院分区：
文献类型：
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作者：
Junzhou Li;Xueying Jia;Jing Zhang;Hongzheng Sun;Quanzhi Zhao
旱稻是一种适应旱作栽培系统的生态型。在本研究中，使用陆地稻基因型 IRAT109 研究了暴露于 15% 聚乙二醇 (PEG) 的发芽种子的发芽特性和转录组的变化。 PEG降低发芽势并抑制种子胚根和胚芽的生长。 PEG胁迫下，赤霉酸（GA）含量下降，脱落酸（ABA）含量增加。转录组分析显示，1270 个基因在胁迫和非胁迫种子之间存在差异表达。在PEG胁迫下，大约56.46%的差异表达基因(DEG)上调，43.54%的DEG下调。基因本体论 (GO) 分析将 1149 个 DEG 分为 52 个功能组，京都基因和基因组百科全书 (KEGG) 分析将 123 个 DEG 分配到 54 个途径。大量与GA信号转导、ABA生物合成与信号转导、防御反应途径相关的DEGs上调。结果表明，GA信号转导和ABA相关基因在干旱胁迫下的发芽过程中发挥着重要作用。这项研究的结果对于了解发芽的分子机制非常有用，并为干直播水稻的分子育种提供候选基因。 © 2019 朋友科学出版社
Upland rice is an ecotype adapted to dry culture system. In this study, changes in germination characteristics and transcriptome of germinating seeds exposed to 15% polyethylene glycol (PEG) were investigated using upland rice genotype IRAT109. PEG reduced germination potential and inhibited growth of seed radicle and plumule. Under PEG stress, gibberellic acid (GA) content decreased and abscisic acid (ABA) content increased. Transcriptome analysis revealed that 1270 genes were differentially expressed between stressed and non-stressed seeds. Approximately, 56.46% of differentially expressed genes (DEGs) were up-regulated and 43.54% of DEGs were down-regulated under PEG stress. Gene Ontology (GO) analysis categorized 1149 DEGs into 52 functional groups and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis assigned 123 DEGs to 54 pathways. A large number of DEGs related to GA signal transduction, ABA biosynthesis and signal transduction, and defense response pathways were upregulated. Results indicated that genes related with GA signal transduction and ABA play important role in germination under drought stress. Findings of this study are highly useful to understand the molecular mechanism of germination and provide candidate genes for molecular breeding in dry direct-seeded rice. © 2019 Friends Science Publishers