An increasing prevalence of non-GII.4 norovirus genotypes in acute gastroenteritis outbreaks in Huzhou, China, 2014-2018
An increasing prevalence of non-GII.4 norovirus genotypes in acute gastroenteritis outbreaks in Huzhou, China, 2014-2018
复制标题
DOI：
10.1007/s00705-020-04599-2
复制
发表时间：
2020-03-27
影响因子：
2.7
通讯作者：
Ji, Lei
中科院分区：
文献类型：
--
作者：
Chen, Liping;Xu, Deshun;Ji, Lei
自 2014 年以来，新型非 GII.4 诺如病毒 (NoV) 基因型继续被报道为全球疫情爆发的主要原因。在这项研究中，我们分析了2014年7月至2018年6月中国湖州新型冠状病毒爆发的流行病学和遗传特征。通过实时 RT PCR 对从 51 次 AGE 爆发中收集的总共 450 份粪便样本进行了 NoV 检测。对 NoV 阳性样品的部分聚合酶和衣壳序列进行扩增和测序，以进行系统发育分析。过去 5 年来，湖州 84.3% 的 AGE 爆发是由 NoV 造成的。大多数新型病毒爆发发生在凉爽的月份（11 月至 3 月），并发生在小学和幼儿园。近年来观察到基因型多样性和优势类型分布的变化。至少鉴定出 8 种基因型，91.9% 的基因型暴发是由非 GII.4 菌株引起的。研究期间流行的前三种基因型是 GII.2[P16]、GII.3[P12] 和 GII.17[P17]。疫情中的主要NoV基因型已从2014-2015年的GII.4变种变为GII.17[P17]，2015-2016年的GII.3[P12]，然后2016-2018年的GII.2[P16]。非 GII.4 NoV 在湖州的疫情中发挥着越来越重要的作用。需要持续监测来监测新病毒株的出现，并帮助控制下一个流行季节的新病毒爆发。
Since 2014, novel non-GII.4 norovirus (NoV) genotypes continue to be reported as the main cause of outbreaks worldwide. In this study, we analyzed the epidemiological and genetic features of NoV outbreaks from July 2014 to June 2018 in Huzhou, China. A total of 450 stool samples collected from 51 AGE outbreaks were tested for NoVs by real-time RT PCR. Partial polymerase and capsid sequences of NoV-positive samples were amplified and sequenced for phylogenetic analysis. NoVs were found to be responsible of 84.3% of AGE outbreaks in Huzhou over the past 5 years. Most NoV outbreaks were reported in the cool months (November-March) and occurred in primary schools and kindergartens. Changes in the diversity of genotypes and the distribution of predominant types were observed in recent years. At least eight genotypes were identified, and 91.9% of the genotyped outbreaks were caused by non-GII.4 strains. The top three circulating genotypes during the study period were GII.2[P16], GII.3[P12], and GII.17[P17]. The predominant NoV genotypes in outbreaks have changed from GII.4 variants to GII.17[P17] in 2014-2015, GII.3[P12] in 2015-2016, and then GII.2[P16] in 2016-2018. Non-GII.4 NoVs play an increasingly important role in outbreaks in Huzhou. Continuous surveillance is needed to monitor the emergence of novel NoV strains and help control NoV outbreaks in the next epidemic season.