Enzyme Instructed Self-assembly Enabled Monomer-excimer Transition to Construct Higher Ordered Luminescent Supramolecular Assembly for Activity-based Bioimaging.
Enzyme Instructed Self-assembly Enabled Monomer-excimer Transition to Construct Higher Ordered Luminescent Supramolecular Assembly for Activity-based Bioimaging.
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DOI：
10.1002/anie.202014278
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发表时间：
2021-01
影响因子：
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通讯作者：
Yuanzhi Zhong;Jie Zhan;Guanghui Xu;Yumiao Chen;Qin Qin-Qin;Xu Liao;Shaodan Ma;Zhimou Yang;
中科院分区：
文献类型：
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作者：
Yuanzhi Zhong;Jie Zhan;Guanghui Xu;Yumiao Chen;Qin Qin-Qin;Xu Liao;Shaodan Ma;Zhimou Yang;
How to construct high-performing excimer-based luminescent analytic tools at low molecular concentrations remains a challenge. Here, we reported that enzyme instructed self-assembly (EISA) enabled the monomer-excimer transition of a coumarin dye ( Cou ) at low molecular concentrations, and the resulting higher ordered luminescent supramolecular assemblies (i.e., nanofibers) efficiently recorded the spatiotemporal details of alkaline phosphatase (ALP) activity in vitro and in vivo . Cou was conjugated to short self-assembly peptides with a hydrophilic ALP-responsive group. By ALP triggering, EISA actuated nanoparticles-nanofibers transition at low peptides concentrations followed by monomer-excimer transition of Cou . Analysis of structure-property relationship revealed that the self-assembly motif was a prerequisite for peptides to induce monomer-excimer transition of Cou . Luminescent supramolecular nanofibers of pYD ( LSN- pYD ) illuminated the intercellular bridge of cancer cells and distinguished cancer cells (tissues) from normal cells (tissues) efficiently and rapidly, promising the potential use for early diagnosis of cancer. This work extends the functions of EISA and provides a new application of supramolecular chemistry.
