INCREASED SURVIVAL OF HEMATOPOIETIC PLURIPOTENT STEM-CELLS INVITRO INDUCED BY A MARROW FIBROBLAST FACTOR
INCREASED SURVIVAL OF HEMATOPOIETIC PLURIPOTENT STEM-CELLS INVITRO INDUCED BY A MARROW FIBROBLAST FACTOR
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DOI：
10.1111/j.1365-2141.1977.tb01004.x
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发表时间：
1977-01-01
影响因子：
6.5
通讯作者：
PATT, HM
中科院分区：
文献类型：
--
作者：
BLACKBURN, MJ;PATT, HM
当小鼠骨髓在骨髓成纤维细胞条件培养液中孵育时，多能干细胞(CFU)的存活显著高于骨髓在新鲜培养液中孵育时的存活。CFU存活率的增加取决于骨髓成纤维细胞培养的年龄、培养中细胞的初始数量和条件培养液的浓度。来自其他成人组织、脾、骨和皮下组织的成纤维细胞条件培养液不能增加CFU的存活率，而胚骨条件培养液能增加CFU的存活率。骨髓成纤维细胞条件培养液对CFU存活率的增加并没有伴随着培养的骨髓有核细胞总数的任何变化，也没有伴随着粒系干细胞(CFUc)或红系干细胞(BFUE)存活率的任何相应增加。骨髓成纤维细胞明显产生一种增加CFU存活率的因子，这可能与骨髓基质在控制造血中的作用有关。
When mouse bone marrow was incubated in medium conditioned by marrow fibroblasts, the survival of pluripotent stem cells (CFUs) was considerably greater than when marrow was incubated in fresh medium. This increase in CFUs survival depended on the age of the marrow fibroblast culture, the initial number of cells in the culture and the concentration of the conditioned medium. Medium conditioned by fibroblasts from other adult tissues, spleen, bone and subcutaneous tissue, did not increase CFUs survival, but medium conditioned by embryo bone did. The increase in CFUs survival by marrow-fibroblast-conditioned medium was not accompanied by any change in the total number of nucleated cells of the incubated marrow nor by any comparable increase in the survival of granulopoietic stem cells (CFUc) or erythropoietic stems cells (BFUE). Marrow fibroblasts apparently produce a factor that increases the survival of CFUs, which may be involved in the role of marrow stroma in the control of hemopoiesis.