Effect of persistent monoarthritis of the temporomandibular joint region on acute mustard oil-induced excitation of trigeminal subnucleus caudalis neurons in male and female rats
Effect of persistent monoarthritis of the temporomandibular joint region on acute mustard oil-induced excitation of trigeminal subnucleus caudalis neurons in male and female rats
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DOI：
10.1016/j.pain.2005.05.013
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发表时间：
2005-09-01
期刊：
影响因子：
7.4
通讯作者：
Bereiter, DF
中科院分区：
文献类型：
--
作者：
Bereiter, DA;Okamoto, K;Bereiter, DF
在雄性和骑自行车的雌性大鼠中，评估了颞下颌关节（TMJ）区域持续性炎症对由急性伤害性刺激相同或对侧TMJ诱发的Fos样免疫反应性（Fos LI）的影响。在完全弗氏佐剂（CFA，25 μ g）引起TMJ炎症后两周，在巴比妥酸盐麻醉下将选择性小纤维刺激剂芥子油（MO，20%）注射到关节炎或对侧TMJ中。MO刺激关节炎颞下颌关节增加Fos-LI同侧，但不是对侧，MO相比，幼稚受试者在三叉神经尾侧亚核/上颈髓（Vc/C-2）交界处的浅层独立的性激素状态。出乎意料的是，MO刺激对关节炎大鼠颞下颌关节也产生了更大的Fos-LI反应同侧MO比幼稚的动物。MO刺激正常颞下颌关节后，在三叉旁背侧区（dPa 5）和Vc/C-2交界处产生的Fos-LI在动情前期显著高于动情间期雌性或雄性单关节炎大鼠。与幼稚动物相比，Fos-LI产生于CFA治疗动物中与MO同侧的Vc/C-2交界处的深层，与先前CFA炎症或性激素状态的部位无关。这些结果表明，颞下颌关节区域的持续性单关节炎增强了三叉神经脑干神经元的兴奋性，随后颞下颌关节损伤，发生双边在多个地区的下三叉神经脑干复合体，并依赖于性激素的状态。(c)2005年国际疼痛研究协会。Elsevier B. V.出版，保留所有权利。
The effect of persistent inflammation of the temporomandibular (TMJ) region on Fos-like immunoreactivity (Fos-LI) evoked by acute noxious stimulation of the same or opposite TMJ was assessed in male and cycling female rats. Two weeks after inflammation of the TMJ by complete Freund's adjuvant (CFA, 25 mu g) the selective small fiber excitant, mustard oil (MO, 20%), was injected into the arthritic or opposite TMJ under barbiturate anesthesia. MO stimulation of the arthritic TMJ increased Fos-LI ipsilateral, but not contralateral, to MO compared to naive subjects in superficial laminae at the trigeminal subnucleus caudalis/upper cervical cord (Vc/C-2) junction independent of sex hormone status. Unexpectedly, MO stimulation of the opposite TMJ in arthritic rats also produced a greater Fos-LI response ipsilateral to MO than naive animals. Fos-LI produced in the dorsal paratrigeminal region (dPa5) and Vc/C-2 junction after MO stimulation of the normal TMJ was significantly greater in proestrous than diestrous females or male monoarthritic rats. In contrast to naive animals, Fos-LI was produced in deep laminae at the Vc/C-2 junction ipsilateral to MO in CFA-treated animals independent of the site of prior CFA inflammation or sex hormone status. These results indicated that persistent monoarthritis of the TMJ region enhanced the excitability of trigeminal brainstem neurons to subsequent TMJ injury that occurred bilaterally in multiple regions of the lower trigeminal brainstem complex and depended on sex hormone status. (c) 2005 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.