Altered distribution of dopaminergic neurons in the brain of L1 null mice
Altered distribution of dopaminergic neurons in the brain of L1 null mice
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DOI：
10.1016/s0165-3806(00)00129-2
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发表时间：
2001-01-31
期刊：
影响因子：
--
通讯作者：
Maness, PF
中科院分区：
文献类型：
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作者：
Demyanenko, GP;Shibata, Y;Maness, PF
小鼠中脑的多巴胺能神经元起源于心室区，沿着放射状胶质细胞径向迁移，然后沿着表达神经细胞粘附分子LI的神经纤维切向迁移，形成黑质（A9组）和腹侧被盖区（VTA）（A10组）。通过酪氨酸羟化酶 (TH) 免疫染色研究 L1 敲除小鼠中 L1 在多巴胺能神经元前体迁移中的作用。在 L1-minus 小鼠的黑质和 VTA 内观察到多巴胺能神经元的头尾分布发生改变。在出生后第 0 天的 L1 缺失小鼠中，Ttl 阳性细胞在背内侧中脑中异常存在，表明迁移受损。从黑质投射到尾壳核的轴突也表现出异常的靶向模式。没有证据表明突变 SN 中多巴胺能细胞丢失。 L1-minus 小鼠中多巴胺能神经元的异常定位在丘脑未定带（A13 组）、下丘脑弓状核（A12）和室周核（A14）中也很明显。野生型小鼠胚胎的黑质、VTA 和下丘脑中的细胞体和轴突表达 L1。这些结果表明，L1 在中脑和间脑多巴胺能神经元细胞群的组织中发挥着重要的发育作用。 (C) 2001 Elsevier Science B.V. 保留所有权利。
Dopaminergic neurons of the mouse mesencephalon originate in the ventricular zone and migrate radially along radial glia then tangentially along nerve fibers that express the neural cell adhesion molecule LI to form the substantia nigra (A9 group) and ventral tegmental area (VTA) (A10 group). The role of L1 in migration of dopaminergic neuronal precursors was investigated in L1 knockout mice by tyrosine hydroxylase (TH) immunostaining. An altered rostrocaudal distribution of dopaminergic neurons was observed within the substantia nigra and VTA of L1-minus mice. In L1 minus mice at postnatal day 0, Ttl-positive cells were present abnormally in the dorsomedial mesencephalon, suggesting impaired migration. Axons projecting from the substantia nigra to the caudate putamen also exhibited an abnormal targeting pattern. There was no evidence of dopaminergic cell loss in the mutant SN. Abnormal localization of dopaminergic neurons in L1-minus mice was also evident in the zona incerta of the thalamus (A13 group), and the arcuate (A12) and periventricular nucleus (A14) of the hypothalamus. Cell bodies and axons in the substantia nigra, VTA, and hypothalamus of wild type mouse embryos expressed L1. These results suggested that L1 plays an important developmental role in the organization of dopaminergic neuronal cell groups in the mesencephalon and diencephalon. (C) 2001 Elsevier Science B.V. All rights reserved.