Rapid modification of bacterial artificial chromosomes by ET-recombination
Rapid modification of bacterial artificial chromosomes by ET-recombination
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DOI：
10.1093/nar/27.6.1555
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发表时间：
1999-03-15
影响因子：
14.9
通讯作者：
Stewart, AF
中科院分区：
文献类型：
--
作者：
Muyrers, JPP;Zhang, YM;Stewart, AF
我们提出了一种修改其宿主菌株中的细菌人造染色体（BAC）的方法。该方法基于通过ET-CLONing通过同源重组。我们通过与含有50 nt的同源性臂的PCR产物重组，成功地在两个不同的基因座上修改了BAC。我们在这里描述的过程很快，发现具有高效率，应适用于所需的任何BAC修饰。
We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.