Alternative splicing of a barley gene results in an excess-tillering and semi-dwarf mutant
Alternative splicing of a barley gene results in an excess-tillering and semi-dwarf mutant
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DOI：
10.1007/s00122-019-03448-4
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发表时间：
2019-10-18
影响因子：
5.4
通讯作者：
Li, Chengdao
中科院分区：
文献类型：
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作者：
Hua, Wei;Tan, Cong;Li, Chengdao
关键信息从大麦EMS诱变突变体中鉴定到一个过量分蘖半矮秆基因Hvhtd，选择性剪接导致了过量分蘖半矮秆性状。分蘖和株高是决定谷类作物株型和产量的重要性状。本研究从EMS处理的大麦群体中鉴定出一个过量分蘖半矮秆突变体(HTD)。对F-1、F-2和F-2：3群体的遗传分析表明，HTD的超分蘖半矮秆由单隐性基因控制。利用BSR-Seq和基因定位技术，将Hvhtd基因定位在2HL染色体上1.8Mb的区间内。将RNA-Seq数据与该区间内的功能基因进行比对，在突变体中发现了一个基因HORVU2Hr1G098820，该基因在外显子2和外显子3之间具有选择性剪接，这是由于外显子和内含子连接处的G到A单核苷酸替换所致。一个表型相似的独立突变体证实了这一结果，并在外显子3和外显子4之间进行了选择性剪接。在这两种情况下，选择性剪接都会导致蛋白质失去功能。HORVU2Hr1G098820基因编码一个胰酶家族蛋白，可能参与IAA信号通路，与绿色革命基因在赤霉酸代谢途径中的作用机制不同。
Key message An excess-tillering semi-dwarf gene Hvhtd was identified from an EMS-induced mutant in barley and alternative splicing results in excess-tillering semi-dwarf traits. Tillering and plant height are important traits determining plant architecture and grain production in cereal crops. This study identified an excess-tillering semi-dwarf mutant (htd) from an EMS-treated barley population. Genetic analysis of the F-1, F-2, and F-2:3 populations showed that a single recessive gene controlled the excess-tillering semi-dwarf in htd. Using BSR-Seq and gene mapping, the Hvhtd gene was delimited within a 1.8 Mb interval on chromosome 2HL. Alignment of the RNA-Seq data with the functional genes in the interval identified a gene HORVU2Hr1G098820 with alternative splicing between exon2 and exon3 in the mutant, due to a G to A single-nucleotide substitution at the exon and intron junction. An independent mutant with a similar phenotype confirmed the result, with alternative splicing between exon3 and exon4. In both cases, the alternative splicing resulted in a non-functional protein. And the gene HORVU2Hr1G098820 encodes a trypsin family protein and may be involved in the IAA signaling pathway and differs from the mechanism of Green Revolution genes in the gibberellic acid metabolic pathway.