VASODILATIVE ACTIVITY IN BLACK FLY SALIVARY-GLANDS
VASODILATIVE ACTIVITY IN BLACK FLY SALIVARY-GLANDS
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DOI：
10.4269/ajtmh.1994.50.241
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发表时间：
1994-02-01
影响因子：
3.3
通讯作者：
CUPP, EW
中科院分区：
文献类型：
--
作者：
CUPP, MS;RIBEIRO, JMC;CUPP, EW
几种含笑属植物的唾液腺提取物。通过皮内注射到兔皮肤的快速和持久的红斑诱导来衡量，表明它们含有血管扩张活性。金黄色葡萄球菌、金黄色葡萄球菌、金黄色葡萄球菌和金黄色葡萄球菌的唾液腺总活性大致相等(滴度为0.03-0.02对腺体)。然而，在高度亲人的物种赭科植物中，总的腺体活动要大一个数量级，只有0.002对腺体就会产生红斑。活性物质的物理稳定性试验表明，这两种植物的血管扩张剂是蛋白质类和热稳定性的。建立了一种两步反相高效液相色谱分离具有相似洗脱模式的两种活性的方法。毛细管凝胶电泳法证实纯化后的蛋白为均一性。苏云金杆菌蛋白质的电喷雾电离质谱仪检测到15,351个道尔顿的质量。TSK高效液相色谱柱中蛋白质洗脱时间的相似性预示着牡蛎和赭草血管扩张蛋白在结构上的一些相似之处。
Salivary gland extracts of several Simulium spp. were shown to contain vasodilative activity as measured by the rapid and persistent induction of erythema in response to intradermal injection into rabbit skin. Total salivary gland activities were approximately equal for S. vittatum, S. metallicum, S. bivittatum, and S. argus (titers of 0.03-0.02 pairs of gland). Total gland activity in the highly anthropophilic species S. ochraceum, however, was an order of magnitude greater, with erythema produced by as little as 0.002 pairs of glands. Tests for physical stability of the activities from two species (S. vittatum and S. ochraceum) indicated that the vasodilators were proteinaceous and heat stable. A two-step, reversed-phase high-performance liquid chromatography (HPLC) procedure was developed that isolated both activities with similar elution patterns. Homogeneity of the purified protein from S. vittatum was confirmed by capillary gel electrophoresis. Electrospray ionization mass spectroscopy of the S. vittatum protein detected a mass of 15,351 daltons. Similarity in elution times of the proteins from a TSK HPLC column predict some structural similarities between the S. vittatum and S. ochraceum vasodilator proteins.