CONDITIONALLY IMMORTALIZED OLIGODENDROCYTE CELL-LINES MIGRATE TO DIFFERENT BRAIN-REGIONS AND ELABORATE MYELIN-LIKE MEMBRANES AFTER TRANSPLANTATION INTO NEONATAL SHIVERER MOUSE BRAINS
CONDITIONALLY IMMORTALIZED OLIGODENDROCYTE CELL-LINES MIGRATE TO DIFFERENT BRAIN-REGIONS AND ELABORATE MYELIN-LIKE MEMBRANES AFTER TRANSPLANTATION INTO NEONATAL SHIVERER MOUSE BRAINS
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DOI：
10.1159/000111284
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发表时间：
1995-05-01
影响因子：
2.9
通讯作者：
CAMPAGNONI, AT
中科院分区：
文献类型：
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作者：
FOSTER, LM;LANDRY, C;CAMPAGNONI, AT
5个永生化的少突胶质细胞系，代表了少突胶质细胞成熟的不同阶段，被移植到新生的shiverer小鼠大脑中，并检查了它们在体内的存活、繁殖和迁移能力。当注射到小鼠前脑时，每一种细胞系都以显著的一致性迁移到大脑的不同区域，这表明这些细胞可能对新生小鼠大脑中存在的不同环境线索做出反应。这些结果与少突胶质细胞谱系中不同阶段的细胞可能具有不同的表面分子和受体的事实相一致。在存活和增殖方面也观察到显著的差异，即使这些细胞系具有非常相似的体外表型。有趣的是，体外表型最成熟的细胞系（N20.1）在移植到大脑后，出现了复杂的膜状突起，让人想起包裹轴突节段的少突胶质细胞。实验表明，这些永生化细胞可作为研究哺乳动物大脑中少突胶质细胞发育、成熟和可能形成髓磷脂的细胞和分子机制的有用模型。
Five immortalized oligodendrocyte cell lines, representing a spectrum of different stages of oligodendrocyte maturation, were transplanted into neonatal shiverer mouse brains and examined for their ability to survive, multiply, and migrate in vivo. Each of the cell lines migrated to different regions of the brain with remarkable consistency when injected into the mouse forebrain, suggesting that the cells might be responding to different environmental cues present in the neonatal mouse brain. These results are consistent with the fact that cells at different stages in the oligodendrocyte lineage probably possess different sets of surface molecules and receptors. Significant differences were also observed in the survival and proliferation of the lines examined, even when the lines tested had quite similar in vitro phenotypes. Interestingly, the cell line with the most mature in vitro phenotype, N20.1, appeared to elaborate membranous processes when transplanted into the brain, reminiscent of oligodendrocytes ensheathing axonal segments. The experiments suggest that these immortalized cells could be useful models to study the cellular and molecular mechanisms involved in the development, maturation and possibly formation of myelin by oligodendrocytes in the mammalian brain.