Rapid intracellular TEA block of the KcsA potassium channel
Rapid intracellular TEA block of the KcsA potassium channel
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DOI：
10.1529/biophysj.104.052043
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发表时间：
2005-02-01
影响因子：
3.4
通讯作者：
Heginbotham, L
中科院分区：
文献类型：
--
作者：
Kutluay, E;Roux, B;Heginbotham, L
在单通道水平上研究了在平面脂质双层中重构的KcsA钾通道中的细胞内四乙基铵（TEA）抑制。TEA作为快速阻断剂（导致电流幅度降低），即使在高带宽下，亲和力也在75 mM范围内。在宽电压范围内的研究表明，TEA块具有复杂的电压依赖性，也取决于离子条件。这些观察的渗透模型的背景下，以扩大我们的理解之间的耦合渗透离子和TEA封锁。
Intracellular tetraethylammonium (TEA) inhibition was studied at the single-channel level in the KcsA potassium channel reconstituted in planar lipid bilayers. TEA acts as a fast blocker (resulting in decreased current amplitude) with an affinity in the 75 mM range even at high bandwidth. Studies over a wide voltage range reveal that TEA block has a complex voltage-dependence that also depends on the ionic conditions. These observations are examined in the context of permeation models to extend our understanding of the coupling between permeant ions and TEA blockade.