GTP-BINDING DOMAIN - 3 CONSENSUS SEQUENCE ELEMENTS WITH DISTINCT SPACING
GTP-BINDING DOMAIN - 3 CONSENSUS SEQUENCE ELEMENTS WITH DISTINCT SPACING
复制标题
DOI：
10.1073/pnas.84.7.1814
复制
发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
MERRICK, WC
中科院分区：
文献类型：
--
作者：
DEVER, TE;GLYNIAS, MJ;MERRICK, WC
通过对9个功能不同的gtp结合蛋白家族的序列比较，进一步了解了gtp结合结构域氨基酸序列的保守性和重要性，包括(i)由三个共识元素GXXXXGK、DXXG和NKXD组成的共识序列，共识间距为40-80或.apprxeq。在第一和第二元素之间有130-170个氨基酸残基。在第二和第三序列元件之间有40-80个氨基酸残基；（ii）在负责碱基特异性的序列元件中，NKXW序列代替NKXD序列允许使用ITP和GTP；（iii） dGTP的使用效率与GTP基本相同；（iv）信号转导蛋白和酶已在9个家族中得到鉴定；(v)当存在多个一致序列元素时，家族保守性允许识别最可能的一致序列元素。利用这些特征，我们筛选了蛋白质鉴定资源的蛋白质序列数据库，并鉴定出除口蹄疫病毒蛋白2C外，已知的gtp结合蛋白与共识序列相匹配。基于这一发现，我们预测口蹄疫病毒蛋白2C与GTP结合，类比地，来自几种相关病毒（脊髓灰质炎病毒、犀牛病毒、脑心肌炎病毒和豇豆花叶病毒）的蛋白2C将与核苷酸结合，作为其生物活性的一部分。
A sequence comparison of nine functionally different GTP-binding protein families has yield further information on the general characterization of the conservation and importance of amino acid sequences in the GTP-binding domain, including (i) a consensus sequence composed of three consensus elements GXXXXGK, DXXG, and NKXD with consensus spacings of either 40-80 or .apprxeq. 130-170 amino acid residues between the first and second elements and .apprxeq. 40-80 amino acid residues between the second and third sequence elements; (ii) the sequence NKXW in place of NKXD in the sequence element responsible for base specificity allows the use of ITP as well as GTP; (iii) dGTP can be used with essentially the same efficiency as GTP: (iv) signal transducing proteins and enzymes have been identified in the nine families; and (v) family conservations allow the identification of the most probable consensus sequence element when more than one is present. Employing these features we have screened the protein sequence data base of the Protein Identification Resource and have identified only known GTP-binding proteins with the exception of protein 2C from foot-and-mouth disease virus as matching the consensus sequence. Based on this finding we predict that foot-and-mouth disease virus protein 2C binds GTP and, by analogy, that protein 2C from several related viruses (polio, rhino, encephalomyocarditis, and cowpea mosaic) will bind a nucleotide as part of its biologic activity.