Ethyl glucuronide in hair and fingernails as a long-term alcohol biomarker
Ethyl glucuronide in hair and fingernails as a long-term alcohol biomarker
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DOI：
10.1111/add.12402
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发表时间：
2014-03-01
期刊：
影响因子：
6
通讯作者：
Lewis, Douglas
中科院分区：
文献类型：
--
作者：
Berger, Lisa;Fendrich, Michael;Lewis, Douglas
目的本研究旨在评价葡萄糖醛酸乙酯（EtG）在头发和指甲中作为长期酒精生物标志物的性能。设计采用概率抽样的横断面调查。背景：美国中西部。参与者是606名年龄在18到25岁之间的大学生，他们被挑选为潜在的研究参与者。采用液相色谱-串联质谱法在三个阈值下测定头发和指甲中的EtG浓度[30 picogram (pg) / mg (mg)；20个pg /毫克;和8pg /mg]。在过去的12周内，任何每周饮酒，增加风险饮酒和高风险饮酒的平均情况都通过参与者访谈进行评估，采用时间线随访法。在所有三个EtG阈值下，高危饮酒组对头发和指甲的敏感性最高[头发：0.43，置信区间(CI) = 0.17, 30 pg/mg时为0.69,0.71,CI = 0.47, 20 pg/mg时为0.95；0.93, CI = 0.79, 8 pg/mg时为1.00；指甲：1.00，在30、20和8 pg/mg时CI = 1.00-1.00]，对任何酒精使用的特异性都最大（头发：1.00，在30和20 pg/mg时CI = 1.00, 1.00；在8 pg/mg时CI = 0.97 -0.99；指甲：1.00，在30、20和8 pg/mg时CI = 1.00-1.00）。受试者工作特征曲线下的指甲EtG浓度显著高于每周饮酒的受试者（P = 0.02， DeLong试验，双尾）和增加饮酒风险的受试者（P = 0.02， DeLong试验，双尾）。结论葡萄糖醛酸乙酯，特别是指甲中的葡萄糖醛酸乙酯，可能有潜力作为酒精使用的定量指标。
Aims This study aimed to evaluate the performance of ethyl glucuronide (EtG) in hair and fingernails as a long-term alcohol biomarker. Design Cross-sectional survey with probability sampling. Setting Midwestern United States. Participants Participants were 606 undergraduate college students between the ages of 18 and 25 years at the time of selection for potential study participation. Measurements EtG concentrations in hair and fingernails were measured by liquid chromatography-tandem mass spectrometry at three thresholds [30 picograms (pg) per milligram(mg); 20 pg/mg; and 8 pg/mg]. Any weekly alcohol use, increasing-risk drinking and high-risk drinking on average during the past 12 weeks was assessed by participant interview using the time-line follow-back method. Findings In both hair and fingernails at all three EtG thresholds, sensitivity was greatest for the high-risk drinking group [hair: 0.43, confidence interval (CI) = 0.17, 0.69 at 30 pg/mg, 0.71, CI = 0.47, 0.95 at 20 pg/mg; 0.93, CI = 0.79, 1.00 at 8 pg/mg; fingernails: 1.00, CI = 1.00-1.00 at 30, 20 and 8 pg/mg] and specificity was greatest for any alcohol use (hair: 1.00, CI = 1.00, 1.00 at 30 and 20 pg/mg; 0.97, CI = 0.92-0.99 at 8 pg/mg; fingernails: 1.00, CI = 1.00-1.00 at 30, 20 and 8 pg/mg). Areas under the receiver operating characteristic curves were significantly higher for EtG concentration in fingernails than hair for any weekly alcohol use (P = 0.02, DeLong test, two-tailed) and increasing-risk drinking (P = 0.02, DeLong test, two-tailed). Conclusions Ethyl glucuronide, especially in fingernails, may have potential as a quantitative indicator of alcohol use.