Preparation, characterization and antioxidant activities of acetylated polysaccharides from Cyclocarya paliurus leaves.
Preparation, characterization and antioxidant activities of acetylated polysaccharides from Cyclocarya paliurus leaves.
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DOI：
10.1016/j.carbpol.2015.07.031
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发表时间：
2015-11
影响因子：
11.2
通讯作者：
Jianhua Xie;Fan Zhang;Zhijun Wang;Mingyue Shen;Shaoping Nie;M. Xie
中科院分区：
文献类型：
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作者：
Jianhua Xie;Fan Zhang;Zhijun Wang;Mingyue Shen;Shaoping Nie;M. Xie
以青钱柳叶多糖为原料，对其进行乙酰化修饰，得到3个乙酰化衍生物Ac-CP 1、Ac-CP 2和Ac-CP 3。研究了乙酰化衍生物的理化性质和抗氧化活性。化学分析和红外光谱分析结果表明，乙酰化衍生物与天然C相比有明显的差异。乙酰化反应成功。相对于未改性的多糖，乙酰化衍生物的蛋白质含量降低，而碳水化合物的值增加。乙酰化衍生物的分子量约为1.05-1.09 × 106 Da，主要由Ara、Gal、Glc、Man、GalA组成。在0.5 mg/ml浓度下，低取代度（0.13 ± 0.01）的Ac-CP 1对DPPH自由基具有较好的抗氧化活性（95.21 ± 0.89%），对β-胡萝卜素-亚油酸也具有较强的螯合活性（34.64 ± 2.07%）。此外，扫描电镜（SEM）观察表明，乙酰化可以改变C.蒲桃叶
In this study, polysaccharides extracted fromCyclocarya paliurusleaves were modified to obtain its three acetylated derivatives, Ac-CP1, Ac-CP2, and Ac-CP3. The physicochemical characteristics and antioxidant activities of acetylated derivatives were investigated. The results of chemical and FT-IR spectrum analysis showed differences between acetylated derivatives and nativeC. paliuruspolysaccharide, which revealed that the acetylation were successful. Relative to unmodified polysaccharide, the protein contents of acetylated derivatives decreased, while carbohydrate values increased. The molecular weight (Mw) of acetylated derivatives were approximately 1.05–1.09 × 106Da and were mainly composed of Ara, Gal, Glc, Man, GalA. Ac-CP1with relatively low degree of substitution (0.13 ± 0.01) exhibited excellent antioxidant activity in DPPH radical assay (95.21 ± 0.89%), and also had strong chelating activity on β-carotene–linoleic acid assay (34.64 ± 2.07%) at 0.5 mg/ml. In addition, scanning electron microscope (SEM) observations suggested that acetylation could change the morphology and structure of polysaccharides fromC. paliurusleaves.