A biocompatible vascularized graphene oxide (GO)-collagen chamber with osteoinductive and anti-fibrosis effects promotes bone regeneration in vivo
A biocompatible vascularized graphene oxide (GO)-collagen chamber with osteoinductive and anti-fibrosis effects promotes bone regeneration in vivo
复制标题
具有骨诱导和抗纤维化作用的生物相容性血管化氧化石墨烯（GO）胶原室可促进体内骨再生
DOI：
10.7150/thno.42006
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发表时间：
2020-01-01
期刊：
影响因子：
12.4
通讯作者：
Wang, Zhenxing
中科院分区：
文献类型：
--
作者：
Fang, Huimin;Luo, Chao;Wang, Zhenxing
移植细胞和组织在骨再生中的存活需要具有充满活力的血管网络的微环境。组织工程室可以在体内提供这一点。然而，常用的硅胶腔是生物惰性的，可引起排异反应和纤维囊。研究表明胶原蛋白具有良好的生物相容性，氧化石墨烯（GO）可以调节体内成骨活性。此外，GO可以与天然可生物降解聚合物交联以构建支架。研究方法：通过将血管穿过包埋的组织工程移植物（成骨诱导的骨髓间充质干细胞-明胶）放置在大鼠腹股沟区，建立血管化的GO-胶原腔模型。采用显微CT扫描、茜素红染色、免疫组化染色等方法评价成骨活性和炎症反应。结果如下：一个月后，体内结果显示，硅胶模型组或无腔室（对照）组的骨矿化和炎症反应显著。血管灌注分析证实，GO-胶原室改善了血管生成过程。EdU标记的细胞显示GO-胶原小室促进了骨髓间充质干细胞的存活和成骨分化。结论：总体而言，新型生物相容性GO-胶原室表现出骨诱导和抗纤维化作用，其改善了体内骨再生。因此，它可以应用于再生医学的其他领域。
The survival of transplanted cells and tissues in bone regeneration requires a microenvironment with a vibrant vascular network. A tissue engineering chamber can provide this in vivo. However, the commonly used silicone chamber is biologically inert and can cause rejection reactions and fibrous capsule. Studies have revealed that collagen is highly biocompatible and graphene oxide (GO) could regulate osteogenic activity in vivo. Besides, GO can be cross-linked with natural biodegradable polymers to construct scaffolds. Methods: A vascularized GO-collagen chamber model was built by placing vessels traversing through the embedded tissue-engineered grafts (osteogenic-induced bone mesenchymal stem cells -gelatin) in the rat groin area. Osteogenic activity and inflammatory reactions were assessed using different methods including micro-CT scanning, Alizarin red staining, and immunohistochemical staining. Results: After one month, in vivo results showed that bone mineralization and inflammatory responses were significantly pronounced in the silicone model or no chamber (control) groups. Vascular perfusion analysis confirmed that the GO-collagen chamber improved the angiogenic processes. Cells labeled with EdU revealed that the GO-collagen chamber promoted the survival and osteogenic differentiation of bone mesenchymal stem cells. Conclusion: Overall, the novel biocompatible GO-collagen chamber exhibited osteoinductive and anti-fibrosis effects which improved bone regeneration in vivo. It can, therefore, be applied to other fields of regenerative medicine.