GENERAL ORGANIZATION OF THE CONJUGAL TRANSFER GENES OF THE INCW PLASMID R388 AND INTERACTIONS BETWEEN R388 AND INCN AND INCP PLASMIDS
GENERAL ORGANIZATION OF THE CONJUGAL TRANSFER GENES OF THE INCW PLASMID R388 AND INTERACTIONS BETWEEN R388 AND INCN AND INCP PLASMIDS
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DOI：
10.1128/jb.172.10.5795-5802.1990
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发表时间：
1990-10-01
影响因子：
3.2
通讯作者：
DELACRUZ, F
中科院分区：
文献类型：
--
作者：
BOLLAND, S;LLOSA, M;DELACRUZ, F
已将IncW质粒R388的完整接合转移基因区克隆到多拷贝载体质粒中，并通过插入诱变定位到连续的14.9-内切酶片段。克隆区的育性仍然可以被共存的IncP质粒抑制。转移区被分成两个区域，一个参与菌毛合成和组装（PILw），另一个参与接合DNA代谢（MOBw）。它们是单独克隆的。PILw还含有参与进入排斥的基因。MOBw含有oriT和PILw有效动员所需的基因产物。MOBw质粒也能被IncN质粒pCU 1的特异性菌毛PILN有效地动员，但不能被IncP质粒RP 1的特异性菌毛PILP有效地动员。
The complete conjugal transfer gene region of the IncW plasmid R388 has been cloned in multicopy vector plasmids and mapped to a contiguous 14.9-kilobase segment by insertion mutagensis. The fertility of the cloned region could still be inhibited by a coresident IncP plasmid. The transfer region has been dissected into two regions, one involved in pilus synthesis and assembly (PILw), and the other involved in conjugal DNA metabolism (MOBw). They have been separately cloned. PILw also contains the genes involved in entry exclusion. MOBw contains oriT and the gene products required for efficient mobilization by PILw. MOBw plasmids could also be mobilized efficiently by PILN, the specific pilus of the IncN plasmid pCU1, but not by PILP, the specific pilus of the IncP plasmid RP1.