CCDC3 is specifically upregulated in omental adipose tissue in subjects with abdominal obesity.
CCDC3 is specifically upregulated in omental adipose tissue in subjects with abdominal obesity.
复制标题
在腹部肥胖受试者的网膜脂肪组织中，CCDC3 特别上调。
DOI：
10.1002/oby.20645
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发表时间：
2014
期刊：
影响因子：
6.9
通讯作者：
Hiroshi Maegawa
中科院分区：
文献类型：
--
作者：
Satoshi Ugi;Shiro Maeda;Yoshihiro Kawamura;Masa-aki Kobayashi;Minako Imamura;Takeshi Yoshizaki;Katsutaro Morino;Osamu Sekine;Hiroshi Yamamoto;Toru Tani;Masatomo Rokushima;Atsunori Kashiwagi;Hiroshi Maegawa
本研究的目的是寻找新的标志物内脏adiposity.MethodsVisceral（网膜）和皮下脂肪组织从43名日本男性。微阵列分析使用总RNA从内脏和皮下脂肪组织获得的五名男子腹部肥胖和五名非肥胖的男子首先进行。结果在评估的30，500个基因中，CCDC3（编码含卷曲螺旋结构域蛋白3）的mRNA表达在腹部肥胖受试者的网膜脂肪组织中上调（3.07倍），但在皮下脂肪组织中没有上调（0.89倍）。在两种不同的肥胖小鼠模型中发现了相似的表达模式。在对所有43名男性的分析中，网膜中的CCDC3mRNA水平与腰围和体重指数呈正相关，而皮下脂肪组织中的CCDC3mRNA水平与腰围和体重指数无关。预测CCDC3是一种分泌蛋白，这是由蛋白质印迹证实，过表达的CCDC3分泌到culture medium.ConclusionsThe表达的CCDC3是专门增加内脏脂肪组织在腹部肥胖的科目。这些结果表明，CCDC3是一个潜在的生物标志物估计内脏肥胖。
ObjectiveThe aim of this study was to search for novel markers of visceral adiposity.MethodsVisceral (omental) and subcutaneous adipose tissues were obtained from 43 Japanese men. Microarray analysis using total RNA from visceral and subcutaneous adipose tissues obtained from five men with abdominal obesity and five nonobese men was first conducted. Then the expression pattern of candidate genes identified in the human study in mouse models of adiposity was examined.ResultsAmong 30,500 genes evaluated, the mRNA expression ofCCDC3(encoding coiled‐coil domain‐containing protein 3) was upregulated in omental adipose tissues from abdominally obese subjects (3.07‐fold) but not in subcutaneous adipose tissues (0.89‐fold). Similar expression patterns were found in two distinct mouse models of obesity. In the analysis of all 43 men,CCDC3mRNA levels in omental, but not in subcutaneous adipose tissue, were positively correlated with waist circumference and body mass index. CCDC3 was predicted to be a secretory protein, which was confirmed by western blotting, as overexpressed CCDC3 was secreted into the culture media.ConclusionsThe expression ofCCDC3is specifically increased in visceral adipose tissues in abdominally obese subjects. These results suggest that CCDC3 is a potential biomarker for estimating visceral adiposity.