Influence of Molecular Sizes of Cryptococcus neoformans Capsular Polysaccharide on Phagocytosis
Influence of Molecular Sizes of Cryptococcus neoformans Capsular Polysaccharide on Phagocytosis
复制标题
新型隐球菌荚膜多糖分子大小对吞噬作用的影响
作者：
A. Yasuoka;S. Kohno;H. Yamada;M. Kaku;H. Koga
本文研究了新生隐球菌荚膜多糖（CPS）对小鼠肺泡巨噬细胞吞噬功能的影响。新生儿血清型A、YC-11、YC-5、YC-27和YC-13。与胎牛血清相比，加入10%小鼠血清后吞噬率显著增加。C.除了YC-27外，观察到新生菌和肺泡巨噬细胞的吞噬作用，YC-27具有薄的荚膜和高毒力。小鼠血清中的吞噬率在YC-11（囊厚度2.8 - 3.5 μm）中为17.3%，在YC-5（囊尺寸0.8 - 1.5 μm）中为39.8%，在YC-27（囊尺寸0.6 - 1.1 μm）中为20.3%，在YC-13（未在显微镜下检测到囊）中为62.8%。使用CL-2B通过凝胶过滤分析的YC-11、YC-5和YC-27的CPS显示接近空隙体积的高分子分数。然而，YC-13的CPS仅显示低分子级分。将广泛洗脱的YC-11的CPS分离成3个级分，并将每个级分加入YC-13的吞噬测定中。吞噬作用显着抑制，特别是通过添加更高的分子级分。这些结果表明，C.肺泡巨噬细胞对新生儿的吞噬作用受CPS分子大小的影响。
The role of capsular polysaccharides (CPS) of Cryptococcus neoformans in phagocytosis by murine alveolar macrophages was investigated in four strains of C. neoformans serotype A, YC‐11, YC‐5, YC‐27 and YC‐13. Phagocytosis rates increased markedly after adding 10% mouse serum, compared to fetal calf serum. The reverse relation between capsular thickness of C. neoformans and phagocytosis by alveolar macrophages was observed except in YC‐27, which had thin capsules and high virulence. The phagocytosis rate in mice serum was 17.3% in YC‐11 (capsule thickness 2.8‐3.5 μm), 39.8% in YC‐5 (capsule size 0.8‐1.5 μm), 20.3% in YC‐27 (capsule size 0.6‐1.1 μm), and 62.8% in YC‐13 (capsule not detected microscopically). The CPS of YC‐11, YC‐5, and YC‐27 analyzed by gel‐filtration using CL‐2B showed high molecular fractions near the void volume. However, the CPS of YC‐13 showed only low molecular fractions. The widely eluted CPS of YC‐11 was separated into 3 fractions and each fraction was added in the phagocytosis assay of YC‐13. Phagocytosis was markedly suppressed particularly by the addition of a higher molecular fraction. These results suggest that phagocytosis of C. neoformans by alveolar macrophages is influenced by the molecular sizes of the CPS.
人肺泡巨噬细胞对新型隐球菌的生长抑制。
DOI：
10.1164/ajrccm/136.5.1242
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Weinberg,PB;Becker,S;Granger,DL;Koren,HS
通讯作者：
Koren,HS
大鼠肺泡巨噬细胞对新型隐球菌的吞噬作用。
DOI：
--
发表时间：
1989
期刊：
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology
影响因子：
--
作者：
Bolanos,B;Mitchell,TG
通讯作者：
Mitchell,TG