Metabolic alterations in leaves of oxalate-rich plant Rumex obtusifolius L. irradiated by gamma rays.
Metabolic alterations in leaves of oxalate-rich plant Rumex obtusifolius L. irradiated by gamma rays.
复制标题
伽马射线照射下富含草酸盐的植物 Rumex obtusifolius L. 叶子的代谢变化。
DOI：
10.1007/s11306-014-0684-4
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发表时间：
2015
期刊：
影响因子：
3.6
通讯作者：
Kawai-Yamada M
中科院分区：
文献类型：
--
作者：
Kitano S;Miyagi A;Oono Y;Hase Y;Narumi I;Yamaguchi M;Uchimiya H;Kawai-Yamada M
某些植物中的草酸盐对脊椎动物有毒，会导致肾衰竭。在本研究中，我们研究了γ射线照射的代谢效率ofRumex obtusifolius，这是已知的积累高水平的可溶性草酸，以降低叶片中草酸的积累。降低发芽率，子叶变色，和变形的真叶观察到提高照射。初级代谢产物的代谢组学分析，使用毛细管电泳-质谱法显示，在植物叶片中的草酸含量从γ射线照射的种子减少。此外，有机酸如苹果酸和2-酮戊二酸也减少，而氨基酸如谷氨酸和谷氨酰胺增加。这些结果表明，种子辐射γ射线导致代谢途径以及植物生长/发育的动态变化。
Oxalate in some plants is toxic to vertebrates and causes kidney failure. In the present study, we investigated on the metabolic effectiveness of gamma ray irradiation to seeds ofRumex obtusifolius,which are known to accumulate high level of soluble oxalate, to lower oxalate accumulation in leaves. Reduced rate of germination, discoloration of cotyledons, and deformed true leaves were observed by elevated irradiation. Metabolome analysis of primary metabolites using capillary electrophoresis–mass spectrometry showed a decrease in oxalate contents in the leaves of plants from gamma ray-irradiated seeds. Moreover, organic acids such as malate and 2-oxoglutarate also decreased, whereas amino acids such as glutamate and glutamine increased. These results indicated that seed irradiation by gamma rays leads to dynamic changes in metabolic pathways as well as plant growth/development.