Vertical transmission of Salmonella Enteritidis with heterogeneous antimicrobial resistance from breeding chickens to commercial chickens in China
Vertical transmission of Salmonella Enteritidis with heterogeneous antimicrobial resistance from breeding chickens to commercial chickens in China
复制标题
中国具有异质耐药性的肠炎沙门氏菌从种鸡到商品鸡的垂直传播
DOI：
10.1016/j.vetmic.2019.108538
复制
发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Wang, Hong-Ning
中科院分区：
文献类型：
--
作者：
Lei, Chang-Wei;Zhang, Yu;Wang, Hong-Ning
由食用被沙门氏菌污染的鸡蛋和鸡肉引起的人类沙门氏菌病已成为全球持续的公共卫生问题。本研究采用全基因组测序技术（WGS）对分离的沙门氏菌进行了亲缘关系和耐药性分析。从一个家禽养殖企业（包括一个种鸡场、一个鸡蛋孵化场和一个商品鸡场）分离的肠道菌株。共148个S。enterica，包括147株S.从2100份粪拭子中分离出肠道菌群，其中种鸡场16株（5.3%），种蛋场38株（4.2%），商品鸡场94株（10.4%）。WGS透露，所有147 S。肠球菌属ST 11型，进一步分为4个不同的核糖体ST和64个核心基因组ST。单核苷酸多态性分型提示存在垂直传播。从种鸡到商品鸡的肠道菌群。对3种不同的耐药质粒（1种携带bla（CTX-M-14）的质粒和2种毒力耐药质粒）进行了鉴定，导致了克隆相关的沙门氏菌的异质性耐药性。肠道菌株。对种鸡场进行常规监测有助于控制沙门氏菌。从农场到餐桌的便利设施。
Human salmonellosis caused by the consumption of eggs and chicken meat contaminated with Salmonella Enteritidis has become a continuing public health concern worldwide. In this study we adopted whole genome sequencing (WGS) to determine the genetic relationship and antimicrobial resistance of S. enterica strains isolated from a poultry breeding enterprise that consists of one breeding chicken farm, one egg hatchery and one commercial chicken farm. A total of 148 S. enterica including 147 S. Enteritidis strains were isolated from 2100 fecal swab samples, with 16 (5.3 %, 16/300) from breeding chicken farm, 38 (4.2 %, 38/900) from egg hatchery and 94 (10.4 %, 94/900) from commercial chicken farm. WGS revealed that all 147 S. Enteritidis strains belonged to ST11, and further divided into 4 different ribosomal STs and 64 core genome STs. Single nucleotide polymorphism typing suggested the presence of the vertical transmission of S. Enteritidis from breeding chicken to commercial chicken. Three different antimicrobial-resistant plasmids including one bla(CTX-M-14)-carrying plasmid and two virulence-resistance plasmids were characterized, resulting in the heterogeneous antimicrobial resistance of clonally related S. Enteritidis strains. Routine surveillance in breeding chicken farms is conducive to the control of S. Enteritidis from farm to fork.