Methodologies for Following EMT In Vivo at Single Cell Resolution.
Methodologies for Following EMT In Vivo at Single Cell Resolution.
复制标题
在单细胞分辨率下体内追踪上皮-间质转化（EMT）的方法。
DOI：
10.1007/978-1-0716-0779-4_23
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
McClay DR
中科院分区：
文献类型：
--
作者：
Massri AJ;Schiebinger GR;Berrio A;Wang L;Wray GA;McClay DR
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An epithelial-mesenchymal transition (EMT) occurs in almost every metazoan embryo at the time mesoderm begins to differentiate. Several embryos have a long record as models for studying an EMT given that a known population of cells enters the EMT at a known time thereby enabling a detailed study of the process. Often, however, it is difficult to learn the molecular details of these model EMT systems because the transitioning cells are a minority of the population of cells in the embryo and in most cases there is an inability to isolate that population. Here we provide a method that enables an examination of genes expressed before, during, and after the EMT with a focus on just the cells that undergo the transition. Single cell RNA-seq (scRNA-seq) has advanced as a technology making it feasible to study the trajectory of gene expression specifically in the cells of interest, in vivo, and without the background noise of other cell populations. The sea urchin skeletogenic cells constitute only 5% of the total number of cells in the embryo yet with scRNA-seq it is possible to study the genes expressed by these cells without background noise. This approach, though not perfect, adds a new tool for uncovering the mechanism of EMT in this cell type.