Effects and mechanisms of iridoid glycosides from Patrinia scabiosaefolia on improving insulin resistance in 3T3-L1 adipocytes
Effects and mechanisms of iridoid glycosides from Patrinia scabiosaefolia on improving insulin resistance in 3T3-L1 adipocytes
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DOI：
10.1016/j.fct.2019.110806
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发表时间：
2019-12-01
影响因子：
4.3
通讯作者：
Kang, Wenyi
中科院分区：
文献类型：
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作者：
Liu, Zhenhua;Xu, Lanting;Kang, Wenyi
胰岛素抵抗引起几种不良反应，如高血压、糖尿病和心血管疾病的不同方面。糙叶败酱菲施是崔佛。是一种传统的中国食用草药，其n-BuOH提取物在12.5 μ M的浓度下显著增加3 T3-L1脂肪细胞的葡萄糖转运。为确定其活性成分，对其化学成分和生物活性进行了研究。两种化合物（1-2）在约25.0 μ M的浓度下可显著改善3 T3-L1脂肪细胞中的胰岛素抵抗（P < 0.001）。化合物2在12.5 μ M时更有效地降低葡萄糖含量（P < 0.001）。化合物1为新化合物，经光谱分析鉴定。蛋白质印迹实验证明化合物1和2诱导p-IRS-1、p-Akt和GLUT 4的上调。因此，我们推测化合物1和2可以通过上调p-IRS-1激活PI 3 K和Akt信号通路，从而在Akt磷酸化之前激活PI 3 K，最终导致GLUT 4易位。这些事件最终改善葡萄糖转运。本研究结果可为开发和有效利用糙叶连翘抗2型糖尿病药物提供科学依据。
Insulin resistance causes several adverse effects such as hypertension, diabetes and different aspects of cardiovascular diseases. Patrinia scabiosaefolia Fisch. ex Trev. is a traditional Chinese edible herbal, whose n-BuOH extract significantly increased glucose transportin 3T3-L1 adipocytes at the concentration of 12.5 mu M. To determine its active constituent, its chemical components and bioactivities were investigated. Two compounds (1-2) could significantly improve insulin resistance in 3T3-L1 adipocytes at concentrations around 25.0 mu M (P < 0.001). Compound 2 was more effective to lower the content of glucose content at 12.5 mu M (P < 0.001). Compound 1 was a new compound identified by spectroscopic methods. Western-blot experiment demonstrated an upregulation of p-IRS-1, p-Akt, and GLUT4 induced by compounds 1 and 2. Hence, we speculated that compounds 1 and 2 could activate PI3K and Akt signaling by up-regulating of p-IRS-1 which resulted in the activation of PI3K before phosphorylating Akt, ultimately led to translocation of GLUT4. These events finally improve glucose transport. Our results may provide the scientific basis for the development and effective use of P. scabiosaefolia against type 2 diabetes.