Oligoclonal expansions of mucosal T cells in Crohn's disease predominate in NKG2D-expressing CD4 T cells
Oligoclonal expansions of mucosal T cells in Crohn's disease predominate in NKG2D-expressing CD4 T cells
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DOI：
10.1038/mi.2013.51
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发表时间：
2014-03-01
影响因子：
8
通讯作者：
Allez, M.
中科院分区：
文献类型：
--
作者：
Camus, M.;Esses, S.;Allez, M.
克罗恩病（CD）是由对肠道微生物的不受控制的免疫应答引起的粘膜肠的炎性病理。在CD患者中已发现T细胞的克隆扩增，表明病原性T细胞的抗原特异性刺激。在这里，我们显示，使用T细胞受体库分析，通过实时PCR，寡克隆扩增发现在CD患者的血液和肠粘膜中的CD8+和CD4+ T细胞。大多数CD4+ T细胞扩增克隆是CD4+NKG2D+ T细胞。这些克隆性扩增在发炎和邻近的健康组织中均被发现，并且在疾病过程中持续存在。在手术切除物的肉眼正常边缘存在这些CD4+NKG2D+ T细胞克隆可能预测术后炎症复发。
Crohn's disease (CD) is an inflammatory pathology of the mucosal intestine that results from uncontrolled immune response towards commensal microbes. Clonal expansions of T cells have been found in patients with CD suggesting an antigen-specific stimulation of pathogenic Tcells. Here we show, using T-cell receptor repertoire analysis by real-time PCR, that oligoclonal expansions are found in both CD8+ and CD4+ T cells in the blood and intestinal mucosa of CD patients. The majority of CD4+ T-cell-expanded clones are CD4+NKG2D+ T cells. These clonal expansions were found in both inflamed and neighboring healthy tissue and were persisting during the course of the disease. The presence of these CD4+NKG2D+ T-cell clones at the macroscopically normal edge of the surgical resection might be predictive of inflammation relapse post surgery.