Different radiosensitive megakaryocytic progenitor cells exist in steady-state human peripheral blood
Different radiosensitive megakaryocytic progenitor cells exist in steady-state human peripheral blood
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DOI：
10.1667/rr3396
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发表时间：
2005-07-01
影响因子：
3.4
通讯作者：
Kuwabara, M
中科院分区：
文献类型：
--
作者：
Kashiwakura, I;Inanami, O;Kuwabara, M
CD 34抗原是一种新的造血干/祖细胞标志物。在本研究中，两个细胞组分，CD 34（低）和CD 34（高），从稳定状态的人外周血的基础上的CD 34抗原表达。对每个细胞组分中所含的集落形成单位巨核细胞（CFU-Meg）进行X射线敏感性和细胞因子作用的比较。CD 34（低）和CD 34（高）细胞组分中CD 34（+）CD 45（+）细胞的含量分别为74.8%和88.8%，CD 34（低）细胞组分中血小板生成素（TPO）支持的CFU-Meg频率是CD 34（高）细胞组分中的1.9倍。CD 34（高）中的CFU-Meg比CD 34（低）中的CFU-Meg更敏感，表明稳态人外周血中含有不同类型的CFU-Meg。而在TPO+细胞因子（粒细胞集落刺激因子G-CSF除外）刺激下，CFU-Meg的辐射存活曲线在细胞组分间无显著差异。TPO + IL-3是X射线照射后两种CFU-Meg存活的最佳组合。本研究还证明TPO + G-CSF能够增加照射的CD 34（低）CFU-Meg的存活。这些结果表明，两个巨核细胞祖细胞群体具有不同的放射敏感性和细胞因子反应被发现在稳态人外周血。(c)2005年，辐射研究协会。
CD34 antigen is a novel marker for human hematopoietic stem/progenitor cells. In the present study, two cell fractions, CD34(low) and CD34(high), were prepared from steady-state human peripheral blood on the basis of CD34 antigen expression. The colony-forming unit megakaryocytes (CFU-Meg) contained in each cell fraction were compared for X-radiation sensitivity and cytokine action. The content of CD34(+)CD45(+) cells in the CD34(low) and CD34(high) cell fractions was 74.8% and 88.8%, respectively, and the frequency of thrombopoietin (TPO)-supported CFU-Meg in the CD34(low) cell fraction was 1.9 times higher than that in CD34(high). The CFU-Meg in CD34(high) were more radiosensitive than those in CD34(low), indicating that steady-state human peripheral blood contains different types of CFU-Meg. However, no significant differences were observed between cell fractions in the radiation survival curves of CFU-Meg stimulated by TPO plus cytokines except granulocyte colony-stimulating factor (G-CSF). TPO plus interleukin 3 was the optimal combination for survival of both types of CFU-Meg after X irradiation. The present study also demonstrated that TPO plus G-CSF is able to increase the survival of irradiated CD34(low) CFU-Meg. These results suggest that two megakaryocytic progenitor populations with different radiosensitivity and cytokine responses are found in steady-state human peripheral blood. (c) 2005 by Radiation Research Society.