Spontaneous reiterations of DNA sequences near the ends of adenovirus type 3 genomes.
Spontaneous reiterations of DNA sequences near the ends of adenovirus type 3 genomes.
复制标题
3 型腺病毒基因组末端附近 DNA 序列的自发重复。
DOI：
10.1016/0042-6822(85)90238-7
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Tibbetts,C
中科院分区：
文献类型：
--
作者：
Larsen,PL;Tibbetts,C
3型腺病毒在HeLa细胞中的反复传代导致了变异基因组的新储备。该原种中的大多数DNA分子的特征在于3型腺病毒基因组图谱左端附近的DNA序列的缺失和取代，如先前报道的（C. C.罗宾逊和C. Tibbetts（1984）Virology 137，276-286）。在这份报告中，变异基因组的表征得到了扩展，并揭示了在病毒DNA的左右两端附近存在病毒DNA序列串联重复的细长DNA分子。证据也支持细胞DNA来源的短插入序列中发现的取代变体。延长的变体是感兴趣的，因为它们的新的重复DNA结构。这些异常序列的位置引发了关于它们对病毒基因表达的潜在影响的问题。
Repeated passage of adenovirus type 3 in HeLa cells has led to a novel stock of variant genomes. Most of the DNA molecules in this stock are characterized by deletions and substitutions of DNA sequences near the left end of the adenovirus type 3 genome map, as reported earlier (C. C. Robinson and C. Tibbetts (1984)Virology137, 276–286). In this report the characterization of the variant genomes is extended and reveals elongated DNA molecules bearing tandem repetitions of viral DNA sequences near the left and right ends of the viral DNA. Evidence is also presented supporting the cellular DNA origin of short insert sequences found in substitution variants. The elongated variants are of interest because of their novel repeated DNA structures. The locations of these aberrant sequences raise questions about their potential impact on viral gene expression.