T-cell specific enhancement of histone H3 acetylation in 5′ flanking region of the IL-2 gene
T-cell specific enhancement of histone H3 acetylation in 5′ flanking region of the IL-2 gene
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DOI：
10.1016/j.bbrc.2005.03.216
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发表时间：
2005-06-03
影响因子：
3.1
通讯作者：
Habu, S
中科院分区：
文献类型：
--
作者：
Wang, L;Kametani, Y;Habu, S
IL-2基因的表达是高度的t细胞特异性的激活依赖的方式。然而，尽管已经确定了相关的转录因子，但对于如何调节t细胞特异性表达知之甚少。为了解决这个问题，我们通过分析IL-2基因转录起始位点上游的组蛋白乙酰化状态来检测IL-2基因的染色质结构变化。有趣的是，在静止的t细胞中，组蛋白乙酰化水平在广泛的上游区域的各个位点都比静止的b细胞高。用PMA和离子霉素刺激t细胞后，组蛋白H3上的相同区域进一步乙酰化。特别是，远端增强子区表现出迅速增强或组蛋白乙酰化，随后是IL-2 mRNA的表达。这些结果表明，在组蛋白H3组成性乙酰化的T细胞中，包括IL-2基因远端增强子区域在内的5'侧翼区域已经可以进入，这可能有助于T细胞特异性IL-2的表达。(c) 2005爱思唯尔公司版权所有。
The IL-2 gene expression is highly T-cell specific in an activation-dependent manner. However, little is known about how T-cell specific expression is regulated, although related transcription factors have been identified. To address this issue, we examined the chromatin structure change of the IL-2 gene by analyzing histone acetylation status in the upstream of IL-2 gene transcription initiation site. Interestingly, the histone acetylation level was found to be higher in various sites on the widespread upstream region in resting T-cells than resting B-cells. After T-cell Stimulation with PMA and ionomycin, the same regions were further acetylated on histone H3. Particularly, the distal enhancer region showed prompt enhancement or histone acetylation, followed by the IL-2 mRNA expression. These results suggest that the 5' flanking region including the distal enhancer region of the IL-2 gene is already accessible in T cells with constitutive acetylation of histone H3,which may serve for T-cell specific IL-2 expression. (c) 2005 Elsevier Inc. All rights reserved.