Functional evaluation of iodoacetic acid induced photoreceptor degeneration in the cat
Functional evaluation of iodoacetic acid induced photoreceptor degeneration in the cat
复制标题
碘乙酸诱导猫光感受器变性的功能评价
DOI：
10.1007/s11427-013-4483-3
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发表时间：
2013-06-01
影响因子：
9.1
通讯作者：
Pu MingLiang
中科院分区：
文献类型：
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作者：
Nan Yan;Zhang Qin;Pu MingLiang
碘乙酸（IAA）已被应用于不同的物种，以急性诱导感光细胞变性。本研究的目的是使用这种毒素彻底消除光感受器并诱导猫完全失明。通过单耳静脉注射（20 mg kg−1）给予IAA。治疗后6个月进行瞳孔对光反射（PLR）、视网膜电图（ERG）、视觉行为检查等功能评价。功能评价后进行形态学检查。目前的结果表明，在施用IAA六个月后，动物失去了视觉功能，完全失明。通过耳静脉注射高剂量IAA建立了一种急性、可靠的猫光感受器完全变性模型，该模型可应用于遗传和细胞治疗以恢复视功能。
Iodoacetic acid (IAA) has been applied to different species to acutely induce photoreceptor degeneration. The purpose of the present study was to use this toxin to thoroughly eliminate photoreceptors and induce complete blindness in the cat. IAA was delivered by single ear vein injection (20 mg kg−1). Six months after the IAA treatment, functional evaluations including pupillary light reflex (PLR), electroretinogram (ERG), visual behavior tests were performed. Morphological examinations were carried out after the functional evaluation. The present result shows that, six months after the IAA application, animals lost visual functions and became completely blind. High dose IAA application via ear vein delivery created an acute and reliable complete photoreceptor degeneration model in the cat. This model can be applied to genetic and cellular therapies for visual function restoration.