Immune functions of the Dscam extracellular variable region in Chinese mitten crab.
Immune functions of the Dscam extracellular variable region in Chinese mitten crab.
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DOI：
10.1016/j.fsi.2023.108850
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发表时间：
2023-05
影响因子：
4.7
通讯作者：
Xiaoli Zhang;Guoqing Shen;Xiao-Na Zhang;Yue Zhao;Weiwei Li;Qun Wang
中科院分区：
文献类型：
--
作者：
Xiaoli Zhang;Guoqing Shen;Xiao-Na Zhang;Yue Zhao;Weiwei Li;Qun Wang
In arthropods, there is only a single copy of Down Syndrome Cell Adhesion Molecule (Dscam) in the genome, but it can exist as numerous splice variants. There are three hypervariable exons in the extracellular domain and one hypervariable exon in the transmembrane domain. In Chinese mitten crab (Eriocheir sinensis), exons 4, 6 and 14 can produce 25, 34 and 18 alternative splice variants, respectively. In this study, through Illumina sequencing, we identified additional splice variants for exons 6 and 14, hence there may be > 50,000 Dscam protein variants. Sequencing of exons 4, 6 and 14 showed that alternative splicing was altered after bacterial stimulation. Therefore, we expressed and purified the extracellular variable region of Dscam (EsDscam-Ig1-Ig7). Exons 4.3, 6.46 and 14.18, three variable exons of the recombinant protein, were randomly selected. The functions ofEsDscam-Ig1-Ig7 in immune defences ofE. sinensiswere subsequently explored.EsDscam-Ig1-Ig7 was discovered to bind to both Gram-positiveStaphylococcus aureusand Gram-negativeVibrio parahaemolyticus, but it did not exhibit antibacterial activity. By promoting hemocyte phagocytosis and bacterial removal,EsDscam-Ig1-Ig7 can also shield the host from bacterial infection. The findings highlight the immunological activities of Dscam alternative splicing and reveal the potential for many more Dscam isoforms than were previously predicted inE. sinensis.
