Selective monitoring of ultra-trace guanine and adenine from hydrolyzed DNA using boron-doped carbon electrode surfaces
Selective monitoring of ultra-trace guanine and adenine from hydrolyzed DNA using boron-doped carbon electrode surfaces
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DOI：
10.1016/j.snb.2020.129192
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发表时间：
2021-01-17
影响因子：
8.4
通讯作者：
Shenashen, Mohamed A.
中科院分区：
文献类型：
--
作者：
Emran, Mohammed Y.;El-Safty, Sherif A.;Shenashen, Mohamed A.
低成本、高灵敏度、高选择性的生物分子超痕量检测电极设计是保证人体健康质量的需要。制备了碳微球（CMS）和硼掺杂碳纳米球（B-CNS）电极，用于DNA水解产物中超痕量鸟嘌呤（Gu）和腺嘌呤（Ad）的检测。B-CNS几何纳米球结构利用了具有多中心结合位点、不同扩散维度、互连和垫表面邻近（即，凹槽、空隙和空腔状巢）。我们的发现表明，B-CNS电极提供了Ad和Gu靶点的自由占据和高扩散率，并具有大量的靶点到位点转运。B-CNS电极实现了高灵敏度和选择性的信号传导的Gu和Ad在痕量水平与低检测限为0.4和0.2 nmol L-1（S/N = 3），分别。B-CNS可从水解DNA中筛选出超痕量浓度的Gu和Ad，具有简单的选择性、高稳定性和多次重复使用性（RSD范围为1.001%-2.73%）。结果表明，B-CNS可用于Gu和Ad的超痕量信号传导，以及评估人类健康质量中的DNA畸变、免疫系统功能障碍和异常突变。
Low cost, sensitive, and selective electrode designs for ultra-trace detection of biomolecules are needed for human health quality. The electrochemical electrode design based on carbon microspheres (CMS), and boron-doped carbon nanospheres (B-CNS) were fabricated for detection and determination of ultra-trace guanine (Gu) and adenine (Ad) from hydrolyzed DNA. The B-CNS geometrical nanosphere structures leverage the electrode designs with multicentral binding sites, diverse diffusion dimensionalities, interlinked and mat surface vicinities (i.e., grooves, voids, and cavity-like nests). Our finding shows evidence that the B-CNS electrode offers free occupation and high diffusivity of Ad and Gu targets with massive target-to-site transport. The B-CNS electrode achieves highly sensitive and selective signaling of Gu and Ad at trace levels with low detection limits of 0.4 and 0.2 nmol L-1 (S/N = 3), respectively. The B-CNS determines the screening of Gu and Ad from hydrolyzed DNA at ultra-trace-level concentrations, facile selectivity, high stability, and multiple reusability (RSD range of 1.001 %-2.73 %). Results showed that B-CNS can be used for ultra-trace signaling of Gu and Ad and evaluation of DNA distortion, immune system dysfunction, and abnormal mutation in the human health quality.