Peripheral Spexin Inhibited Food Intake in Mice
Peripheral Spexin Inhibited Food Intake in Mice
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DOI：
10.1155/2020/4913785
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发表时间：
2020-08-05
影响因子：
2.8
通讯作者：
Wang, Xinchun
中科院分区：
文献类型：
--
作者：
Lv, Shuangyu;Zhou, Yuchen;Wang, Xinchun
Spexin (SPX, NPQ) 是一种新型内源性神经肽，首次通过生物信息学鉴定。 Spexin基因和蛋白广泛分布于中枢神经系统和周围组织，如下丘脑和消化道。 spexin 在哺乳动物食欲调节中的作用尚不清楚。本研究旨在探讨外周spexin对小鼠食物摄入的机制和影响。在光照期间，腹膜内 (i.p.) 注射 spexin (10 nmol/小鼠) 显着抑制禁食小鼠治疗后 2、4 和 6 小时的累积食物摄入量。在黑暗时期，Spexin（1 和 10 nmol/小鼠，腹腔注射）显着抑制自由进食小鼠治疗后 4 和 6 小时的累积食物摄入量。 GALR3拮抗剂SNAP37889，而非GALR2拮抗剂，显着拮抗spexin诱导的累积食物摄入量（0-6小时）的抑制作用。 Spexin 显着降低下丘脑中 NpymRNA 的 mRNA 水平，而不是 Agrp、Pomc、Cart、Crh、Orexin 或 Mch。 Spexin（10 nmol/小鼠，腹腔注射）增加了下丘脑 AHA 和 SCN 中 c-Fos 阳性神经元的数量，但在 ARC、DMN、LHA、PVN、SON 或 VMH 中没有增加。 spexin 上调下丘脑 p-CaMK2 蛋白表达。这项研究表明，急性外周注射 spexin 会抑制小鼠的食物摄入。厌食作用可能是由 GALR3 介导的，并通过下丘脑中的 p-CaMK2 和 c-Fos 抑制神经肽 Y (NPY)。
Spexin (SPX, NPQ), a novel endogenous neuropeptide, was firstly identified by bioinformatics. Spexin gene and protein widely distributed in the central nervous system and peripheral tissues, such as the hypothalamus and digestive tract. The role of spexin in appetite regulation in mammalian is still unclear. The present study was designed to investigate the mechanism and effect of peripheral spexin on food intake in mice. During the light period, an intraperitoneal (i.p.) injection of spexin (10 nmol/mouse) significantly inhibited cumulative food intake at 2, 4, and 6 h after treatment in fasted mice. During the dark period, spexin (1 and 10 nmol/mouse, i.p.) significantly suppressed cumulative food intake at 4 and 6 h after treatment in freely feeding mice. The GALR3 antagonist SNAP37889, not GALR2 antagonist, significantly antagonized the inhibitory effect on cumulative food intake (0-6 h) induced by spexin. Spexin significantly reduced the mRNA level ofNpymRNA, notAgrp,Pomc,Cart,Crh,Orexin, orMch, in the hypothalamus. Spexin (10 nmol/mouse, i.p.) increased the number of c-Fos positive neurons in hypothalamic AHA and SCN, but not in ARC, DMN, LHA, PVN, SON, or VMH. The hypothalamic p-CaMK2 protein expression was upregulated by spexin. This study indicated that acute peripheral injection of spexin inhibited mouse food intake. The anorectic effect may be mediated by GALR3, and inhibiting neuropeptide Y (NPY) via p-CaMK2 and c-Fos in the hypothalamus.