Recognition-mediated particle detection under microfluidic flow with waveguide-coupled 2D photonic crystals: towards integrated photonic virus detectors.
Recognition-mediated particle detection under microfluidic flow with waveguide-coupled 2D photonic crystals: towards integrated photonic virus detectors.
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DOI：
10.1039/c7lc00221a
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发表时间：
2017-05-02
期刊：
影响因子：
6.1
通讯作者：
Miller BL
中科院分区：
文献类型：
--
作者：
Baker JE;Sriram R;Miller BL
与标准CMOS制造方法兼容的无标记生物检测方案构成了一个重要目标，因为这些是用于大规模生产高灵敏度生物传感器的工具。二维平板光子晶体（2D slab-PhC）传感器已被假定为超高灵敏度的检测组件，但到目前为止，在流动下的病毒或模拟物的光介导检测尚未得到证实。我们报告的设计和优化的一个新的W1波导耦合的2D平板PhC传感器，具有非常适合病毒检测的几何形状。用荧光乳胶颗粒进行的概念验证实验证实，传感器可以响应单个颗粒在空气中和含水覆盖层下的渗透。随后的抗体功能化传感器和病毒模拟物的实验证实了该设备通过免疫介导的过程在流动下检测病毒大小的颗粒的能力。这项工作为将2D平板PhC传感器纳入完全集成的光子传感器系统奠定了基础。
Label-free biodetection schemes compatible with standard CMOS fabrication methods constitute an important goal, as these are enabling tools for the mass production of high-sensitivity biosensors. Two-dimensional slab photonic crystal (2D slab-PhC) sensors have been posited as ultrahigh-sensitivity detection components, but to date recognition-mediated detection of viruses or simulants under flow has not been demonstrated. We report the design and optimization of a new W1 waveguide-coupled 2D slab-PhC sensor, with a geometry well suited to virus detection. Proof of concept experiments with fluorescent latex particles verified that the sensor could respond to infiltration of a single particle, both in air and under an aqueous cover layer. Subsequent experiments with antibody-functionalized sensors and virus simulants confirmed the ability of the device to detect virus-sized particles under flow via a recognition-mediated process. This work sets the stage for incorporation of 2D slab-PhC sensors into fully integrated photonic sensor systems.