Activation of DNA enzyme 10-23 by tethering an intercalator to its backbone.
Activation of DNA enzyme 10-23 by tethering an intercalator to its backbone.
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DOI：
10.1093/nass/nrl083
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发表时间：
2006-01-01
期刊：
影响因子：
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通讯作者：
Asanuma, Hiroyuki
中科院分区：
文献类型：
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作者：
Hayashi, Hiroyuki;Liang, Xingguo;Asanuma, Hiroyuki
通过共价引入偶氮苯、蒽醌、2-芪唑和芘等嵌入剂，大大提高了RNA切割DNA酶10-23的活性。嵌入剂通过酰胺键连接到 D-苏氨醇，后者通过典型的亚磷酰胺化学插入 DNA 的主链。仅当嵌入剂系在催化环和 3' 侧结合臂之间的连接点时，才能观察到裂解活性的增加。引入的嵌入剂本身以及用于束缚它的连接体的结构极大地影响了裂解活性。蒽醌是我们研究的最有效的激活剂，与天然 DNA 酶相比，其活性增加了约八倍。
The activity of RNA cleaving DNA enzyme 10-23 is greatly improved by covalently introducing an intercalator, such as azobenzene, anthraquinone, 2-stilbazole, and pyrene. The intercalator is attached via an amide bond to D-threoninol, which inserts into the backbone of DNA with typical phosphoramidite chemistry. The increase of cleavage activity is observed only when an intercalator is tethered at the junction point between the catalytic loop and the binding arm at 3' side. Structures of the introduced intercalator itself as well as the linker for tethering it greatly influence the cleavage activity. In the case of anthraquinone, the most efficient activator we investigated, about eight folds of activity increase are obtained in comparison with the native DNA enzyme.