Shifts of sediment bacterial community and respiration along a successional gradient in a typical karst plateau lake wetland (China)
Shifts of sediment bacterial community and respiration along a successional gradient in a typical karst plateau lake wetland (China)
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DOI：
10.1007/s00343-020-0073-y
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发表时间：
2020-10
影响因子：
1.6
通讯作者：
Pinhua Xia;Jian Zhang;Jinbo Liu;Lifei Yu
中科院分区：
文献类型：
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作者：
Pinhua Xia;Jian Zhang;Jinbo Liu;Lifei Yu
细菌是湖泊碳循环的重要调节者，也是沉积物生态系统过程的中心。然而，沉积物微生物群落及其对湖泊湿地演替的呼吸响应却知之甚少。在这项研究中，我们采集了贵州高原草海湿地(中国)四个不同演替点(光芒草带、三叶草带、三叶草带和灯心草带)的沉积物样品。使用高通量测序方法研究了这些传代点的细菌群落。在野外采用静态箱测定沉积物微生物呼吸(SR)，在实验室测定底泥基础呼吸(BR)。结果表明，底泥中的优势细菌种类为变形杆菌(34.7%)、绿藻(17.8%)、类杆菌(7.3%)、酸性细菌(6.6%)和蓝藻(6.1%)。主坐标分析表明，沿演替梯度不同采样点的微生物群落结构差异较大，表明细菌群落结构对湖泊湿地演替较为敏感。不同的水文状况和土壤特性，如Fe2+、Mn2+和沉积物有机碳(SOC)含量，可能是造成草海湿地不同演替阶段沉积物微生物特征差异的重要因素。此外，还发现从TEP开始，SR显著增加。卢森斯带给J。而BR的反应则相反。细菌群落结构沿演替梯度的变化可能是造成沉积物呼吸作用差异的主要原因。
Bacteria are important regulators of carbon cycling in lakes and are central to sediment ecosystem processes. However, the sediment microbial communities and their respiratory responses to the lake wetland succession are poorly understood. In this study, we collected sediment samples from four different succession points (thePotamogeton lucenszone, theScirpus tabernaemontanizone, theScirpus triqueterzone, and theJuncus effususzone) in the Caohai Wetland of the Guizhou Plateau (China). The bacterial communities at these succession points were studied using a high-throughput sequencing approach. The sediment microbial respiration (SR) was measured using static chambers in the field and basal respiration (BR) was determined in the laboratory. The results show that the dominant bacterial taxa in the sediment was Proteobacteria (34.7%), Chloroflexi (17.8%), Bacteroidetes (7.3%), Acidobacteria (6.6%), and Cyanobacteria (6.1%). Principal coordinate analysis showed that the microbial community structure differs significantly at different sampling points along the successional gradient, indicating that the bacterial community structure is sensitive to the lake wetland succession. Different hydrological regimes and soil characteristics such as, Fe2+, Mn2+, and sediment organic carbon (SOC) content may be important factors responsible for the differences in the sediment microbial characteristics of the different successional stages in the Caohai wetland. Additionally, it was found that the SR increased significantly from theP. lucenszone to theJ. effususzone, but BR had the opposite response. The shifts in the bacterial community structure along the successional gradient may be the main reason for the observed differences in sediment respiration.