Overexpression of OsZHD1, a zinc finger homeodomain class homeobox transcription factor, induces abaxially curled and drooping leaf in rice
Overexpression of OsZHD1, a zinc finger homeodomain class homeobox transcription factor, induces abaxially curled and drooping leaf in rice
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DOI：
10.1007/s00425-013-2009-7
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发表时间：
2014-04-01
期刊：
影响因子：
4.3
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Xu, Yang;Wang, Yihua;Wan, Jianmin
卷叶是水稻的一个重要农艺性状，近年来受到广泛关注。然而，对水稻卷叶的分子机制，特别是离轴卷叶的分子机制研究甚少。我们从T-1转基因水稻中发现了一个新的叶片背面卷曲和下垂的显性突变体。与插入的转移DNA共分离的背面卷曲的叶表型是由锌指同源异型域类同源异型盒转录因子（OsZHD 1）的过表达引起的。OsZHD 1在野生型植株中表现出组成型表达模式，并在发育中的叶片和穗中积累。OsZHD 1或其最接近的同源物OsZHD 2的人工过表达诱导远轴叶卷曲。组织学分析表明，叶片泡状细胞数量的增加和排列的异常是造成叶片背面卷曲的原因。本研究报道了OsZHD 1基因在水稻形态建成中的重要作用，特别是在叶片卷曲调控中的作用，为水稻叶片发育的分子机制提供了新的视角。
Leaf rolling is receiving considerable attention as an important agronomic trait in rice (Oryza sativa L.). However, little has been known on the molecular mechanism of rice leaf rolling, especially the abaxial rolling. We identified a novel abaxially curled and drooping leaf-dominant mutant from a T-1 transgenic rice line. The abaxially curled leaf phenotypes, co-segregating with the inserted transferred DNA, were caused by overexpression of a zinc finger homeodomain class homeobox transcription factor (OsZHD1). OsZHD1 exhibited a constitutive expression pattern in wild-type plants and accumulated in the developing leaves and panicles. Artificial overexpression of OsZHD1 or its closest homolog OsZHD2 induced the abaxial leaf curling. Histological analysis indicated that both the increased number and the abnormal arrangement of bulliform cells in leaf were responsible for the abaxially curled leaves. We herein reported OsZHD1 with key roles in rice morphogenesis, especially in the modulating of leaf rolling, which provided a novel insight into the molecular mechanism of leaf development in rice.