Nitric oxide negatively regulates gibberellin signaling to coordinate growth and salt tolerance in Arabidopsis
Nitric oxide negatively regulates gibberellin signaling to coordinate growth and salt tolerance in Arabidopsis
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DOI：
10.1016/j.jgg.2022.02.023
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发表时间：
2022-08-24
影响因子：
5.9
通讯作者：
Zuo, Jianru
中科院分区：
文献类型：
--
作者：
Chen, Lichao;Sun, Shuhao;Zuo, Jianru
为了应对动态变化的环境，植物必须很好地协调生长和应激反应之间的平衡。然而，基本的监管机制在很大程度上仍然难以捉摸。植物激素赤霉素通过蛋白酶体降解DELLA转录抑制因子的抑制机制促进生长。相反，应激诱导的一氧化氮（NO）爆发增强了应激耐受性，主要依赖于NO介导的s-亚硝基化，这是一种基于氧化还原的翻译后修饰。在这里，我们发现拟南芥RGA蛋白（DELLAs的一个关键成员）中Cys-374的s-亚硝基化抑制了它与F-box蛋白SLY1的相互作用，从而阻止了它在盐度条件下的蛋白酶体降解。因此，RGA的积累延缓了生长，但增强了耐盐性。我们认为NO通过RGA的s -亚硝基化负调控赤霉素信号，以协调生长和逆境反应的平衡。版权所有(C) 2022，作者中国科学院遗传与发育生物学研究所，中国遗传学会。爱思唯尔有限公司和科学出版社出版。
In response to dynamically altered environments, plants must finely coordinate the balance between growth and stress responses for their survival. However, the underpinning regulatory mechanisms remain largely elusive. The phytohormone gibberellin promotes growth via a derepression mechanism by proteasomal degradation of the DELLA transcription repressors. Conversely, the stress-induced burst of nitric oxide (NO) enhances stress tolerance, largely relying on NO-mediated S-nitrosylation, a redox-based posttranslational modification. Here, we show that S-nitrosylation of Cys-374 in the Arabidopsis RGA protein, a key member of DELLAs, inhibits its interaction with the F-box protein SLY1, thereby preventing its proteasomal degradation under salinity condition. The accumulation of RGA consequently retards growth but enhances salt tolerance. We propose that NO negatively regulates gibberellin signaling via S-nitrosylation of RGA to coordinate the balance of growth and stress responses when challenged by adverse environments. Copyright (C) 2022, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press.