Effect of feeding regime on the metabolism of ochratoxin a during the in vitro incubation in buffered rumen fluid from cows
Effect of feeding regime on the metabolism of ochratoxin a during the in vitro incubation in buffered rumen fluid from cows
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DOI：
10.1080/17450390109381984
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发表时间：
2001-12
期刊：
影响因子：
--
通讯作者：
H. Müller;K. Müller;H. Steingass
中科院分区：
文献类型：
--
作者：
H. Müller;K. Müller;H. Steingass
在采自两头断奶奶牛的瘤胃液缓冲液中添加纯赭曲霉毒素A(OA)，在厌氧条件下孵化。这两只动物都被喂以六种饲料，其中包括青草、青贮或干草，以及两种不同数量的大麦和豆粕精料。每只动物4次孵化，连续几天饲喂。在所考察的所有条件下，OA的浓度呈指数下降到很低或检测不到的水平，半衰期为0.51h到2.76h，并伴随着赭曲霉毒素α(Oα)的出现，相对于OA的消失，Oα的平均生成量接近100%，与饮食和动物无关。基于每只动物和日粮4次孵化，OA消失率受两只动物瘤胃液来源的影响(P<；0.1)，以及基础成分的类型和饲料中精料的数量，以及这些因素之间的交互作用。鲜草替代青贮料或干草，精料含量由干物质的10%提高到50%，可加速OA的大部分消解(P<；0.1)。由此可见，瘤胃对OA的解毒能力不受OA产量的限制，而是强烈依赖于动物和日粮。
Pure ochratoxin A (OA) was added to buffered rumen fluid collected from two fisrulated cows and incubated under anaerobic conditions. Both animals were fed six diets containing grass, grass silage or hay, and two different amounts of concentrate consisting of barley and soybean meal. Four incubations per animal and diet were carried out at consecutive days. The concentration of OA declined exponentially to a very low or non‐detectable level under all conditions examined, with half‐lives at 0.51 to 2.76 h. The disappearance of OA was accompanied by the appearance of ochratoxin α (Oα) with an average amount of Oα formed relative to the disappearance of OA near 100%, independent of diet and animal. Based on four incubations per animal and diet the rate of OA disappearance was affected (P < 0.1) by the origin of rumen fluid from two animals, as well as by the type of basic component and amount of concentrate in the diet, with interactions between these factors. The disappearance of OA mostly was accelerated (P<0.1) by replacing grass silage or hay by fresh grass and by increasing the content of concentrate from 10 to 50% of dry matter. It is concluded that the capacity of the rumen to detoxify OA is not limited by the yield of Oa from OA but is strongly dependent on animal and diet.