Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length
Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length
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DOI：
10.1126/science.1154819
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发表时间：
2008-06-06
期刊：
影响因子：
56.9
通讯作者：
Ishikawa, Fuyuki
中科院分区：
文献类型：
--
作者：
Miyoshi, Tomoichiro;Kanoh, Junko;Ishikawa, Fuyuki
端粒是保护染色体末端的特殊染色质结构。端粒保护蛋白1（Pot 1）与端粒富含G的突出端结合，从而保护端粒并调节端粒酶.哺乳动物POT 1和TPP 1相互作用并构成六蛋白shelterin复合物的一部分。在这里，我们报告说，TPZ 1，TPP 1同源裂变酵母，形成一个复杂的Pot 1。Tpz 1结合到Ccq 1和以前未发现的蛋白Poz 1（Pot 1相关的裂殖酵母），保护端粒冗余和调节端粒酶的积极和消极的方式，分别。因此，Pot 1-Tpz 1复合物通过募集效应分子Ccq 1和Poz 1来实现其功能。此外，Poz 1桥接Pot 1-Tpz 1和Taz 1-Rap 1，从而连接单链和双链端粒DNA区域.这种分子结构与哺乳动物shelterin的分子结构相似，表明整个DNA-蛋白质结构在进化过程中是保守的。
Telomeres are specialized chromatin structures that protect chromosomal ends. Protection of telomeres 1 ( Pot1) binds to the telomeric G- rich overhang, thereby protecting telomeres and regulating telomerase. Mammalian POT1 and TPP1 interact and constitute part of the six- protein shelterin complex. Here we report that Tpz1, the TPP1 homolog in fission yeast, forms a complex with Pot1. Tpz1 binds to Ccq1 and the previously undiscovered protein Poz1 ( Pot1- associated in Schizosaccharomyces pombe), which protect telomeres redundantly and regulate telomerase in positive and negative manners, respectively. Thus, the Pot1- Tpz1 complex accomplishes its functions by recruiting effector molecules Ccq1 and Poz1. Moreover, Poz1 bridges Pot1- Tpz1 and Taz1- Rap1, thereby connecting the single- stranded and double- stranded telomeric DNA regions. Such molecular architectures are similar to those of mammalian shelterin, indicating that the overall DNA- protein architecture is conserved across evolution.