The role of mucopolysaccharides in vesicle architecture and endothelial transport. An electron microscope study of myocardial blood vessels.
The role of mucopolysaccharides in vesicle architecture and endothelial transport. An electron microscope study of myocardial blood vessels.
复制标题
DOI：
10.1083/jcb.52.1.198
复制
发表时间：
1972-01
期刊：
影响因子：
--
通讯作者：
Cohen AS
中科院分区：
文献类型：
--
作者：
Shirahama T;Cohen AS
电子显微镜研究有助于确定胞饮小泡的精细结构，并提供信息，从而更好地了解其功能（1-3）。尽管预测了在跨内皮转运中发挥作用的”毛细血管内层（或鞘）”（4），但大多数电子显微镜研究并未清楚地证明这种结构（1-3），直到引入了溴化铵红（RR）染色方法（5）。Luft描述了毛细血管和毛细血管内层的RR染色超微结构（6），并指出RR在某些条件下穿透胞饮囊泡，染料缓慢扩散到组织中，通常不穿透质膜。由于其最终产品的细粒度和相对可控的电子不透明度，RR染色在我们看来可能允许良好的电子显微镜分辨率与示踪技术相结合。本研究的目的是（a）分析胞饮囊泡的精细结构与囊泡结构中粘多糖的关系，以及（B）更好地理解表面涂层在内皮运输中的作用。
Electron microscope studies have helped to define the fine structure of pinocytic vesicles and have provided information leading to a better understanding of their function (1-3). Although" an endocapillary layer (or sheath)" which would play a role in transendothelial transport was predicted (4), most electron microscope studies did not clearly demonstrate such a structure (1-3) until the ruthenium-red (RR) staining method was introduced (5). Luft described the RR-stained ultrastructure of blood capillary and endocapillary layers (6) and pointed out that RR penetrates pinocytic vesicles under certain conditions and that the dye diffuses into tissue slowly and does not usually penetrate the plasma membrane. Because of the fine granularity and the relatively controllable electron opacity of its end product, the RR stain seemed to us potentially to allow good electron microscope resolution in combination with tracer techniques. The purpose of the present study was (a) to analyse the fine structure of pinocytic vesicles in relation to mucopolysaccharides in the vesicle architecture, and (b) to obtain a better understanding of the role of the surface coating in endothelial transport.