Point mutations in acetylcholinesterase 1 associated with chlorpyrifos resistance in the brown planthopper, Nilaparvata lugens Stal
Point mutations in acetylcholinesterase 1 associated with chlorpyrifos resistance in the brown planthopper, Nilaparvata lugens Stal
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乙酰胆碱酯酶 1 的点突变与褐飞虱 Nilaparvata lugens Stal 对毒死蜱的抗性相关
DOI：
10.1111/imb.12309
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发表时间：
2017
影响因子：
2.6
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Zhang Y;Yang B;Li J;Liu M;Liu Z
杀虫剂耐药性通常是由于靶位点不敏感造成的，例如乙酰胆碱酯酶 (AChE) 的点突变对有机磷酸酯和氨基甲酸酯产生耐药性。通过毒死蜱的九代连续选择，从田间产生的主要水稻害虫褐飞虱种群中获得了抗性种群（R9）。通过不使用任何杀虫剂的饲养，还从同一田间种群中构建了相对易感种群（S9）。与敏感菌株Sus[中等致死量(LC50) = 0.012 mg/l]相比，R9的耐药率(RR)为253.08倍，而S9的RR仅为2.25倍。胡椒基丁醚和磷酸三苯酯在 R9 中对毒死蜱的协同作用不到三倍，表明高耐药性的其他重要机制。 R9 和 S9 之间粗 AChE 的关键特性差异支持了靶位点不敏感性。与S9相比，R9个体和野外种群的NlAChE1中检测到了3个突变（G119S、F331C和I332L），但NlAChE2中未检测到突变。 G119S和F331C可以降低重组NlAChE1的杀虫剂敏感性，而I332L通过增加F331C对靶标不敏感性的影响来发挥作用。 F331C 可能是有害的，因为它会影响 NlAChE1 的催化效率，而 I332L 会减少这些不利影响并维持 AChE 的正常功能。
Insecticide resistance frequently results from target‐site insensitivity, such as point mutations in acetylcholinesterases (AChEs) for resistance to organophosphates and carbamates. From a field‐originated population ofNilaparvata lugens, a major rice pest, a resistant population (R9) was obtained by nine‐generation continuous selection with chlorpyrifos. From the same field population, a relatively susceptible population (S9) was also constructed through rearing without any insecticides. Compared to the susceptible strain, Sus [medium lethal dose (LC50) = 0.012 mg/l], R9 had a resistance ratio (RR) of 253.08‐fold, whereas the RR of S9 was only 2.25‐fold. Piperonyl butoxide and triphenyl phosphate synergized chlorpyrifos in R9 less than three‐fold, indicating other important mechanisms for high resistance. The target‐site insensitivity was supported by the key property differences of crude AChEs between R9 and S9. Compared to S9, three mutations (G119S, F331C and I332L) were detected in NlAChE1 from individuals of the R9 and field populations, but no mutation was detected in NlAChE2. G119S and F331C could decreased insecticide sensitivities in recombinant NlAChE1, whereas I332L took effect through increasing the influence of F331C on target insensitivity. F331C might be deleterious because of its influence on the catalytic efficiency of NlAChE1, whereas I332L would decrease these adverse effects and maintain the normal functions of AChEs.