Differential expression of cyclin-dependent kinase inhibitors and apoptosis-related proteins in endocervical lesions
Differential expression of cyclin-dependent kinase inhibitors and apoptosis-related proteins in endocervical lesions
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DOI：
10.1016/j.ejca.2007.06.019
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发表时间：
2007-09-01
影响因子：
8.4
通讯作者：
Herrington, C. Simon
中科院分区：
文献类型：
--
作者：
El-Ghobashy, Alaa A.;Shaaban, Abeer M.;Herrington, C. Simon
肿瘤的发生与细胞周期调控和细胞凋亡的异常有关。本研究采用免疫组织化学方法检测了91例腺宫颈病变中细胞周期蛋白依赖性激酶抑制因子(CDKI)和细胞凋亡相关蛋白(p16、p21、p53、BC12和Hsp27)的表达。P21和p53在正常宫颈(n=11)、子宫内膜异位症/管状子宫内膜样化生(n=19)、宫颈腺体上皮内瘤变(CGIN)/原位腺癌(n=33)和浸润性腺癌(n=28)的表达均显著升高。P16在CGIN/AIS和浸润性腺癌中呈弥漫性强表达，在子宫内膜异位症的部分病变中呈局灶性表达，在正常宫颈中无表达。BC12在子宫内膜异位症组织中的表达高于CGIN/AIS和腺癌。P16免疫组织化学染色能准确区分宫颈肿瘤性病变和非肿瘤性病变，前提是弥漫性强阳性。同样，BC12的弥漫性表达区分了子宫内膜异位症/子宫内膜异位症/子宫内膜异位症和CGIN/AIS。这些数据表明，CDKI和细胞凋亡相关蛋白的分析为宫颈腺性病变的诊断评估提供了有用的信息。(C)2007爱思唯尔有限公司。保留所有权利。
The development of neoplasia is associated with abnormalities of cell cycle control and apoptosis. In this study, a panel of cyclin-dependent kinase inhibitors (CDKIs) and apoptosis-related proteins (p16, p21, p53, Bc12 and hsp27) was analysed by immunohistochernistry in 91 glandular cervical lesions. A significant increase in p21 and p53 expression occurred from normal cervix (n = 11) through endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19) and cervical glandular intraepithelial neoplasia (CGIN)/adenocarcinoma in situ (AIS) (n = 33) to invasive adenocarcinoma (n = 28). p16 showed diffuse strong expression in CGIN/AIS and invasive adenocarcinoma compared with focal expression in some TEW endometriosis lesions and no expression in normal cervix. Bc12 was highly expressed in TEM/endometriosis compared with CGIN/AIS and adenocarcinoma. p16 immunostaining discriminated accurately between neoplastic and non-neoplastic cervical lesions, provided that diffuse strong positivity was present. Similarly, diffuse expression of Bc12 distinguished endometriosis/TEM from CGIN/AIS. These data demonstrate that analysis of CDKIs and apoptosis-related proteins provides useful information in the diagnostic assessment of glandular lesions of the cervix. (C) 2007 Elsevier Ltd. All rights reserved.