Milk Oligosaccharides Inhibit Human Rotavirus Infectivity in MA104 Cells
Milk Oligosaccharides Inhibit Human Rotavirus Infectivity in MA104 Cells
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DOI：
10.3945/jn.116.246090
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发表时间：
2017-09-01
影响因子：
4.2
通讯作者：
Ramani, Sasirekha
中科院分区：
文献类型：
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作者：
Laucirica, Daniel R.;Triantis, Vassilis;Ramani, Sasirekha
背景：牛奶中的寡聚核苷酸作为可溶性诱饵受体，防止病原体粘附到婴儿肠道。牛奶低聚糖降低猪轮状病毒株的感染性;然而，对人类轮状病毒的影响还不太清楚。目的：在这项研究中，我们确定了特异性和丰富的牛奶低聚糖对2种全球优势的人类轮状病毒株的感染性的影响。方法：四种牛奶低聚糖-2 '-岩藻糖基乳糖（2' FL）、3 '-唾液酸乳糖（3' SL）、6 '-唾液酸乳糖（6' SL）、通过非洲绿色猴肾上皮细胞上的荧光焦点试验，测试了它们对人轮状病毒G1 P [8]和G2 P [4]感染性的影响（MA 104细胞）。在感染性测定中的不同时间点加入寡聚核苷酸。感染的情况下，低聚糖作为controls.Results：当与感染的情况下，聚糖相比，所有低聚糖大大降低了两种人轮状病毒株在体外的感染性，但是，病毒株特异性差异的影响进行了观察。与对照感染相比，当在感染开始后加入2 'FL时，观察到G1 P [8]感染性的最大降低（62%降低，P < 0.01），而当在感染期间加入3' SL +6 'SL的混合物时，观察到G2 P [4]感染性的最大降低（73%降低，P < 0.01）。与单独的3 'SL（减少47%）或6' SL（减少40%）相比，与母乳中存在的比例相同的3 'SL +6' SL的混合物在降低G2 P [4]感染性方面更有效（减少73%，P < 0.01）。对于所有的低聚糖的感染性的降低是介导的病毒的影响，而不是对cells.Conclusions：牛奶低聚糖降低人轮状病毒在MA 104细胞的感染性，主要是通过对病毒的影响。虽然母乳喂养的婴儿受到直接保护，但在婴儿配方奶粉中添加特定的低聚糖可能会给配方奶粉喂养的婴儿带来这些益处。
Background: Oligosaccharides in milk act as soluble decoy receptors and prevent pathogen adhesion to the infant gut. Milk oligosaccharides reduce infectivity of a porcine rotavirus strain; however, the effects on human rotaviruses are less well understood.Objective: In this study, we determined the effect of specific and abundant milk oligosaccharides on the infectivity of 2 globally dominant human rotavirus strains.Methods: Four milk oligosaccharides-2'-fucosyllactose (2'FL), 3'-sialyllactose (3'SL), 6'-sialyllactose (6'SL), and galacto-oligosaccharides-were tested for their effects on the infectivity of human rotaviruses G1P[8] and G2P[4] through fluorescent focus assays on African green monkey kidney epithelial cells (MA104 cells). Oligosaccharides were added at different time points in the infectivity assays. Infections in the absence of oligosaccharides served as controls.Results: When compared with infections in the absence of glycans, all oligosaccharides substantially reduced the infectivity of both human rotavirus strains in vitro; however, virus strain-specific differences in effects were observed. Compared with control infections, the maximum reduction in G1P[8] infectivity was seen with 2'FL when added after the onset of infection (62% reduction, P < 0.01), whereas the maximum reduction in G2P[4] infectivity was seen with the mixture of 3'SL + 6'SL when added during infection (73% reduction, P < 0.01). The mixture of 3'SL + 6'SL at the same ratio as is present in breast milk was more potent in reducing G2P[4] infectivity (73% reduction, P < 0.01) than when compared with 3'SL (47% reduction) or 6'SL (40% reduction) individually. For all oligosaccharides the reduction in infectivity was mediated by an effect on the virus and not on the cells.Conclusions: Milk oligosaccharides reduce the infectivity of human rotaviruses in MA104 cells, primarily through an effect on the virus. Although breastfed infants are directly protected, the addition of specific oligosaccharides to infant formula may confer these benefits to formula-fed infants.