Quantitative determination of deoxyribonucleic acid from cells collected on filters.
Quantitative determination of deoxyribonucleic acid from cells collected on filters.
复制标题
定量测定过滤器上收集的细胞中的脱氧核糖核酸。
DOI：
10.1016/0003-2697(80)90485-6
复制
发表时间：
1980
影响因子：
2.9
通讯作者：
R. Wagner
中科院分区：
文献类型：
--
作者：
S. Williams;A. Sasaki;M. A. Matthews;R. Wagner
描述了一种从三醋酸纤维素膜过滤器上收集的哺乳动物细胞中定量细胞脱氧核糖核酸的方法。基于抗肿瘤抗生素米霉素的DNA依赖性荧光增强，通过超声从细胞中释放的DNA进行荧光定量。在超声之前用牛血清白蛋白预处理过滤器是必要的，以防止dna -米霉素荧光的损失。离体毛细血管内皮细胞DNA含量为7.1 pg /细胞，肝细胞DNA含量为17.7 pg /细胞。
A method is described for the quantitation of cellular deoxyribonucleic acid from mammalian cells collected on cellulose triacetate membrane filters. DNA released from cells by sonication is fluorescently quantitated based on the DNA-dependent fluorescence enhancement of the anti-tumor antibiotic mithramycin. Pretreatment of filters with bovine serum albumin prior to sonication is necessary to prevent the loss of DNA-mithramycin fluorescence. The cellular DNA content in isolated capillary endothelium is 7.1 pg per cell and in isolated hepatocytes, 17.7 pg per cell.