Methods for in vitro percutaneous absorption studies IV: The flow-through diffusion cell.
Methods for in vitro percutaneous absorption studies IV: The flow-through diffusion cell.
复制标题
体外经皮吸收研究方法IV：流通扩散池。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
R. Stewart
中科院分区：
文献类型：
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作者：
R. Bronaugh;R. Stewart
开发了用于经皮吸收研究的流通扩散池系统。报告了有限数量化合物的初步研究结果。细胞由特氟隆制成，底部有一个玻璃窗，用于观察受体内容物。为了获得准确的结果，通过接收器（体积，0.4 mL）的流速至少为5 mL/h。在流通池和标准静态扩散池中测定水、可的松和苯甲酸的皮肤渗透。两种细胞的吸收曲线和定量值相似。在大鼠体内以及在流通池和静态扩散池中使用大鼠皮肤测定了应用于凡士林载体中的可的松和苯甲酸的渗透。在体内和体外程序的结果之间获得了良好的一致性。与静态细胞相比，疏水化合物[3-苯基-2-丙烯基2-氨基苯甲酸酯（邻氨基苯甲酸肉桂酯）]的经皮吸收在流通细胞中与生理盐水受体溶液一起增强。使用6%的非离子表面活性剂聚乙二醇20油基醚（PEG-20油基醚）的水溶液，使用任一细胞获得最大体外吸收。
A flow-through diffusion cell system for percutaneous absorption studies has been developed. The results of initial studies with a limited number of compounds are reported. The cells were constructed from Teflon and contained a glass window in the bottom for viewing the receptor contents. A flow rate of at least 5 mL/h is required through the receptor (volume, 0.4 mL) for accurate results. The skin permeation of water, cortisone, and benzoic acid was determined in the flow-through cell and a standard static-diffusion cell. The absorption profiles and quantitative values obtained were similar for the two types of cells. The permeation of cortisone and benzoic acid applied in a petrolatum vehicle was determined in vivo in rats and with rat skin in the flow-through and static-diffusion cells. Good agreement was obtained between the results of the in vivo and in vitro procedures. The percutaneous absorption of a hydrophobic compound [3-phenyl-2-propenyl 2-aminobenzoate (cinnamyl anthranilate)] was enhanced with normal saline receptor solution in the flow-through cell when compared with results in the static cell. Maximum in vitro absorption was obtained with either cell using a 6% solution in water of the nonionic surfactant polyethylene glycol 20 oleyl ether (PEG-20 oleyl ether).