Strain and Age Effects on Aqueous Humor Dynamics in the Mouse
Strain and Age Effects on Aqueous Humor Dynamics in the Mouse
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DOI：
10.1167/iovs.15-16720
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发表时间：
2015-09-01
影响因子：
4.4
通讯作者：
Clark, Abbot F.
中科院分区：
文献类型：
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作者：
Millar, J. Cameron;Phan, Tien N.;Clark, Abbot F.
目的。我们评估了年轻组和老年组中几种小鼠品系的房水动力学（AHD）差异。方法。对白化小鼠 (A/J、BALB/cJ) 和色素小鼠 (C3H/HeJ、C57-BL/6J) 小鼠（年轻 [2(1/2)-4(1/2) 个月] 和老年 [10-12 个月]）进行了研究。测量眼内压（IOP）。在插管眼中，评估巩膜外静脉压（Pe）（血液回流）。评估其他 AHD 参数（流出设施 [C]、房水形成率 [Fin]）（恒流输注）。葡萄膜巩膜流出率(Fu)通过使用修正的Goldmann方程计算(Fu((calc)))获得，并且在另外的眼睛中(用于比较)通过FITC-葡聚糖灌注(Fu((FITC-dex)))获得。结果。色素菌株的眼压较高，但除 C57-BL/6J 菌株外，不表现出年龄依赖性。在 BALB/cJ（向下箭头 83.3%）、C3H/HeJ（向下箭头 78.0%）和 C57-BL/6J（向下箭头 85.0%）菌株中，Fu((calc)) 随着年龄的增长而降低。在 A/J 菌株中，Fu((calc)) 随着年龄的增长而下降（向下箭头 70.0%），但不显着。在 C3H/HeJ（向下箭头 53.6%）菌株中，Fin 随着年龄的增长而减少。在 C57-BL/6J 和 A/J 品系中，Fin 随着年龄的增长而降低，但不显着。 BALB/cJ 菌株中的 C 随着年龄的增长而增加（向上箭头 62.5%）。在C3H/HeJ和C57-BL/6J菌株中，C随着年龄的增长而增加，但不显着。巩膜外静脉压范围为 6.0 至 6.6 mm Hg（白化株）至 8.5 至 8.9 mm Hg（色素株）。 Pe 不依赖于年龄，但在有色素的动物中较高。结论。在小鼠中，Fu 和 Fin 随着年龄的增长而减弱。随着动物进入中年，C 往往会增加。 Fu、IOP、C、Fin 和 Pe 存在应变差异。目前的研究结果为不同研究报告中不同菌株之间的比较提供了重要基础。
PURPOSE. We evaluated differences in aqueous humor dynamics (AHD) among several mouse strains within younger and older age groups.METHODS. Albino (A/J, BALB/cJ) and pigmented (C3H/HeJ, C57-BL/6J) mice (young [2(1/2)-4(1/2) months] and aged [10-12 months]) were studied. Intraocular pressure (IOP) was measured. In cannulated eyes, episcleral venous pressure (Pe) was assessed (blood reflux). Other AHD parameters (outflow facility [C], aqueous humor formation rate [Fin]) were assessed (constant flow infusion). Uveoscleral outflow rate (Fu) was obtained by calculation (Fu((calc))) using the modified Goldmann equation, and in additional eyes (for comparison), by FITC-dextran perfusion (Fu((FITC-dex))).RESULTS. Intraocular pressure was higher in pigmented strains, but did not exhibit age-dependence, except in the C57-BL/6J strain. Fu((calc)) decreased with age in BALB/cJ (down arrow 83.3%), C3H/HeJ (down arrow 78.0%), and C57-BL/6J (down arrow 85.0%) strains. In the A/J strain, Fu((calc)) decreased with age (down arrow 70.0%), but not significantly. Fin decreased with age in the C3H/HeJ (down arrow 53.6%) strain. In C57-BL/6J and A/J strains, Fin decreased with age, but not significantly. C in the BALB/cJ strain increased with age (up arrow 62.5%). In C3H/HeJ and C57-BL/6J strains, C increased with age, but not significantly. Episcleral venous pressure ranged from 6.0 to 6.6 mm Hg (albino strains) to 8.5 to 8.9 mm Hg (pigmented strains). Pe was not age dependent, but was higher in pigmented animals.CONCLUSIONS. In mouse, Fu and Fin diminish with age. C tends to increase as animals progress to middle life. There are strain differences in Fu, IOP, C, Fin, and Pe. The current findings provide an important foundation for comparisons among different strains in different study reports.