Development of a novel ELISA for detection of anti-A and anti-B antibodies in recipients of ABO-incompatible living donor liver grafts
Development of a novel ELISA for detection of anti-A and anti-B antibodies in recipients of ABO-incompatible living donor liver grafts
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DOI：
10.1620/tjem.211.359
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Satomi, Susumu
中科院分区：
文献类型：
--
作者：
Satoh, Akefumi;Kawagishi, Naoki;Satomi, Susumu
ABO血型不合（ABO-I）肝移植受者的早期经验使其存活率明显低于ABO血型相合受者。因此，建立准确、快速的抗A、抗B抗体（Abs）检测方法，对预防ABO-Ⅰ型肝移植中的体液排斥反应具有重要意义。凝集滴度法一直是测定这些抗体的标准方法，但结果的解释存在偏倚。在这里，我们已经开发了一个客观和定量的酶联免疫吸附试验（ELISA）来测量抗A和抗B抗体。该试验仅需要少量（10 μ l）受体血清。我们应用新研制的ELISA法对活体肝移植受者进行了监测，并探讨了ELISA法与凝集效价的相关性。Abs的斯皮尔曼相关系数范围为0.461 - 0.812。在1例体液排斥反应中，ELISA法检测到抗体的增加比凝集法早1天。总之，我们的ELISA方法被证明是有用的，以检测抗A和抗B抗体滴度的增加在早期阶段，从而有助于及时治疗由于ABO-I体液排斥反应。
The survival rate in ABO-incompatible (ABO-I) liver transplantation was much lower than that in ABO-compatible recipients for the early experiences. It is therefore essential to develop the precise and fast measurement of anti-A and anti-B antibodies (Abs) to prevent humoral rejection in ABO-I liver transplantation. Agglutination titer has been the standard method to measure these Abs, but the interpretation of the results is subject to bias. Here, we have developed an objective and quantitative enzyme-linked immunosorbent assay (ELISA) to measure anti-A and anti-B Abs. This test requires only a small amount (10 mu 1) of recipient's serum. We applied the newly developed ELISA to monitor living donor liver transplant recipients and investigated the correlation between ELISA and agglutination titer. The Spearman's correlation coefficient for Abs ranged from 0.461 to 0.812. Moreover, in one case of humoral rejection, the increase of Abs was detected by ELISA one day earlier than by the agglutination titer. In conclusion, our ELISA method proved useful to detect an increase of anti-A and anti-B Abs titers at an early stage, thereby contributing to a prompt treatment of humoral rejection due to ABO-I.